Description

Bz-Dab(Nbd)-Ala-Trp-Phe-Pro-Pro-Nle-Nh2 CAS NO 161238-74-0 is a synthetic, custom-designed peptide sequence featuring a benzoyl (Bz) group and a nitrobenzoxadiazole (Nbd) fluorescent tag on the diaminobutyric acid (Dab) residue. This compound is engineered for advanced biochemical research, offering a valuable tool for studying protease activity, enzyme kinetics, and receptor-ligand interactions due to its specific cleavage sites and fluorescent properties. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on drug discovery and proteomics.

Application

• Protease Substrate & Inhibitor Screening: Acts as a specific, fluorogenic substrate for studying the activity and inhibition profiles of proteolytic enzymes like prolyl oligopeptidase.
• Biochemical Assay Development: Serves as a key component in developing high-throughput fluorescence-based assays for drug discovery pipelines.
• Enzyme Kinetics Studies: Used to determine kinetic parameters (Km, Vmax, kcat) of target enzymes in real-time due to its Nbd fluorescence upon cleavage.
• Cell Biology & Imaging: Employed in cellular uptake studies and intracellular protease localization experiments.
• Peptide-Based Drug Research: Provides a model compound for investigating peptide stability, metabolism, and structure-activity relationships (SAR).
• Academic & Pharmaceutical R&D: A critical reagent for fundamental research in neurobiology, oncology, and metabolic disorders where specific proteases are therapeutic targets.

Basic Information

Product Name	Bz-Dab(Nbd)-Ala-Trp-Phe-Pro-Pro-Nle-Nh2
CAS No.	161238-74-0
Molecular Formula	C58H68N12O12
Molecular Weight	1101.2 g/mol
Synonyms	Benzoyl-Dab(NBD)-Ala-Trp-Phe-Pro-Pro-Nle-amide; NBD Fluorescent Peptide Substrate; Bz-Dab(NBD)-AWFPPNle-NH2; 7-Nitrobenz-2-oxa-1,3-diazol-4-yl-labeled Peptide; Fluorogenic Peptide Substrate; Prolyl Oligopeptidase Substrate; NBD-Peptide; Custom Fluorescent Peptide 161238-74-0
EINECS	Contact for details

Quality Control

Every batch of Bz-Dab(Nbd)-Ala-Trp-Phe-Pro-Pro-Nle-Nh2 is manufactured under strict quality management systems and undergoes comprehensive analytical characterization to ensure identity, purity, and performance. Our products are supplied with a detailed Certificate of Analysis (COA) that includes data from HPLC, MS, and NMR analyses. We support research and development with materials that meet the high standards required for reproducible scientific results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical mass
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Solubility	Soluble in DMSO, DMF; slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.