Description

Ac-Tyr(Po3H2)-Glu-Glu-Ile-Glu CAS NO 159439-02-8 is a synthetic, phosphorylated pentapeptide derivative of significant interest in biochemical research and pharmaceutical development. This compound serves as a critical tool for studying signal transduction pathways, particularly those involving tyrosine phosphorylation and SH2 domain interactions. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in kinase/phosphatase assay development, antibody production, and targeted drug discovery.

Application

• Biochemical Research Reagent: Used as a specific substrate or inhibitor in enzymatic assays for protein tyrosine kinases (PTKs) and phosphatases (PTPs).
• Antigen & Immunogen: Serves as a hapten for generating and screening phospho-specific antibodies for Western blotting, ELISA, and immunohistochemistry.
• Cell Signaling Studies: Employed to investigate intracellular signaling cascades, protein-protein interactions, and SH2 domain binding affinity.
• Diagnostic Development: Incorporated into diagnostic kits and platforms for detecting kinase activity or phospho-protein levels in clinical and research samples.
• Pharmaceutical Screening: Acts as a key component in high-throughput screening (HTS) assays to identify and characterize novel kinase-targeted therapeutic compounds.
• Peptide Library Component: Used in the synthesis of focused peptide libraries for proteomics and epitope mapping studies.

Basic Information

Item	Details
Product Name	Ac-Tyr(Po3H2)-Glu-Glu-Ile-Glu
CAS No.	159439-02-8
Molecular Formula	C34H50N6O19P2
Molecular Weight	932.75 g/mol
Synonyms	Acetyl-O-phosphono-L-tyrosyl-L-α-glutamyl-L-α-glutamyl-L-isoleucyl-L-α-glutamic acid; Acetyl-phosphotyrosine-glutamyl-glutamyl-isoleucyl-glutamic acid; Ac-pYEEIE; pYEEIE (acetylated); Phosphotyrosine Peptide (pYEEIE); EGFR-derived Phosphopeptide (pY1068); SH2 Domain Ligand Peptide; EGFR (pY1068) Peptide
EINECS	Contact for details

Quality Control

Every batch of Ac-Tyr(Po3H2)-Glu-Glu-Ile-Glu is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We guarantee high purity and batch-to-batch consistency suitable for sensitive research applications. Full analytical documentation, including a detailed Certificate of Analysis (COA) with HPLC purity, MS confirmation, and amino acid analysis data, is provided to ensure traceability and compliance with your research standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Single Phosphorylation (MS)	Present on Tyrosine residue
Water Content (KF)	≤ 5.0%
Peptide Content (AAA)	≥ 80%
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.