Description

Sepharose Fast Flow Sp is a high-performance, beaded agarose-based chromatography medium designed for high-speed, high-resolution cation exchange separations. Its robust cross-linked structure ensures excellent chemical and physical stability, making it ideal for demanding industrial-scale purification processes. This product is essential for the downstream processing of biomolecules, including monoclonal antibodies, recombinant proteins, and enzymes, serving critical needs in the biopharmaceutical, vaccine production, and life sciences research sectors.

Application

• Industrial-scale capture and intermediate purification of therapeutic proteins and monoclonal antibodies.
• High-resolution polishing steps in downstream bioprocessing to remove aggregates, fragments, and host cell proteins.
• Purification of vaccines, plasma proteins, and other biological products under Good Manufacturing Practice (GMP) conditions.
• Process development and optimization in biopharmaceutical research and pilot-scale production.
• Separation of enzymes, peptides, and nucleic acids in life sciences research laboratories.
• Integration into continuous and simulated moving bed (SMB) chromatography systems for enhanced productivity.

Basic Information

Product Name	Sepharose Fast Flow Sp
CAS No.	159251-40-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sepharose FF Sp; Sepharose Fast Flow SP; SP Sepharose Fast Flow; Sulfopropyl Sepharose Fast Flow; Cation Exchange Sepharose Fast Flow; Agarose-based SP ion exchanger; Fast Flow Cation Exchange Resin; SPFF
EINECS	Contact for details

Quality Control

Our Sepharose Fast Flow Sp is manufactured under a strict quality management system. Each lot undergoes rigorous testing to ensure consistent performance, including assessments of ligand density, particle size distribution, flow properties, and dynamic binding capacity. Certificates of Analysis (COA) are provided with each shipment, detailing specifications relevant to chromatography applications. Production adheres to principles suitable for use in GMP-regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store the suspension in 20% ethanol at 4°C to 30°C. Do not freeze. For long-term storage, ensure the resin remains fully submerged in the storage solution to prevent drying and microbial growth.

Specification

Item	Specification
Matrix	Cross-linked 6% agarose beads
Ligand	Sulfopropyl (SP) strong cation exchanger
Particle Size	45 - 165 μm (mean 90 μm)
Dynamic Binding Capacity*	> 80 mg human IgG/ml resin
pH Stability	2 - 14 (long term); 3 - 12 (working)
Maximum Pressure	0.3 MPa (3 bar, 44 psi)
Maximum Flow Rate	> 700 cm/h
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.

*Typical value measured under defined conditions. Contact us for specific protein binding data.