Description

(D-Val22)-Big Endothelin-1 Fragment (16-38) (Human) CAS NO 158884-64-1 is a synthetic, modified peptide fragment derived from the Big Endothelin-1 precursor protein. This compound is a critical research tool for investigating the structure-activity relationships and processing mechanisms of endothelin, a potent vasoconstrictor. It is primarily utilized by researchers in pharmaceutical R&D and academic biochemistry laboratories focused on cardiovascular disease, signal transduction, and peptide hormone metabolism.

Application

• Biochemical Research: Used as a reference standard and substrate in enzymatic studies of endothelin-converting enzyme (ECE) activity and specificity.
• Structure-Activity Relationship (SAR) Studies: The D-amino acid substitution at position 22 makes it a valuable probe for analyzing peptide conformation, receptor binding, and metabolic stability.
• Pharmaceutical Development: Serves as a key intermediate or tool compound in the discovery and development of novel cardiovascular therapeutics targeting the endothelin system.
• Analytical Method Development: Employed as a calibrant or control in high-performance liquid chromatography (HPLC) and mass spectrometry (MS) methods for peptide analysis.
• Cell Signaling Studies: Applied in in vitro assays to study the biosynthesis, processing, and biological effects of endothelin peptides.

Basic Information

Product Name	(D-Val22)-Big Endothelin-1 Fragment (16-38) (Human)
CAS No.	158884-64-1
Molecular Formula	C115H179N33O34S4
Molecular Weight	2632.1 g/mol
Synonyms	Big ET-1 (16-38), [D-Val22]-Big Endothelin-1 (16-38); Human Big Endothelin-1 Fragment (16-38) with D-Valine at position 22; [D-Val22]-preproendothelin-1 (103-125); ET-1 (1-21) Precursor Fragment; Big Endothelin-1 (Leu6-Ser39) [D-Val22] analog; H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-D-Val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser-OH (Disulfide bridge: Cys1-Cys15)
EINECS	Contact for details

Quality Control

Our peptide products are synthesized and purified under controlled GMP-like conditions to ensure batch-to-batch consistency and high purity. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and Mass Spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, amino acid analysis, and other relevant specifications are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep the lyophilized powder at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption. Solutions should be prepared in appropriate buffers and used immediately or stored aliquoted at ≤ -20°C for short-term use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Amino Acid Composition	Within ± 10% of theoretical values
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Acetate Content (HPIC)	≤ 10.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.