Description

Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-Nh2 is a synthetic, modified peptide sequence containing a 7-methoxycoumarin-4-acetyl (Mca) fluorophore and a 2,4-dinitrophenyl (Dnp) quencher group. This compound is specifically engineered as a highly sensitive and selective fluorogenic substrate for the study of matrix metalloproteinase (MMP) activity, particularly MMP-2 and MMP-9. It is an essential research tool for pharmaceutical R&D, academic biochemistry, and diagnostic development laboratories focused on cancer metastasis, tissue remodeling, and inflammatory diseases.

Application

• Fluorogenic Protease Assays: Primary substrate for the continuous, real-time kinetic measurement of MMP-2 (Gelatinase A) and MMP-9 (Gelatinase B) enzymatic activity.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential MMP inhibitor compounds for oncology and anti-inflammatory therapeutic development.
• Biochemical Research: Fundamental studies on protease kinetics, enzyme specificity, and the role of MMPs in physiological and pathological processes.
• Diagnostic Development: Used in the development of in vitro diagnostic assays to detect and quantify MMP activity in biological samples (e.g., serum, tissue lysates).
• Cell-Based Studies: Application in live-cell imaging and assays to monitor localized MMP activity and invasion in cancer cell models.
• Quality Control Reagent: Serves as a reference standard and critical reagent for validating the performance of commercial MMP assay kits and related research products.

Basic Information

Product Name	Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-Nh2
CAS No.	158584-08-8
Molecular Formula	C78H104N20O19S
Molecular Weight	1641.85 g/mol
Synonyms	Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Alternative sequence reference); MMP-2/MMP-9 Fluorogenic Substrate; Mca-PLGL-Dpa-AR-NH2>; (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(2,4-dinitrophenyl)-NH2; Fluorogenic Peptide Substrate for Gelatinases; MMP Substrate IV; CAS 158584-08-8
EINECS	Contact for details

Quality Control

Every batch of our Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-Nh2 is manufactured and analyzed under strict quality management systems. We guarantee high purity and consistent performance, with specifications verified by advanced analytical techniques including HPLC, MS, and NMR. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity is provided with each shipment to ensure it meets the rigorous standards required for critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical mass
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Functional Activity	≥ 90% relative activity vs. control
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.