Description

H-Ala-D-Phe-Ala-Oh is a synthetic tripeptide featuring a D-phenylalanine residue, which confers unique conformational and biological stability. This structural motif is critical for developing peptide-based pharmaceuticals and research tools with enhanced resistance to enzymatic degradation. It serves as a valuable building block for medicinal chemists and researchers in the pharmaceutical, biotechnology, and life science sectors.

Application

• Pharmaceutical Intermediate: A key chiral building block for the synthesis of novel therapeutic peptides and peptidomimetics.
• Protease Substrate/Analogue: Used in biochemical research to study enzyme kinetics, specificity, and inhibition, particularly for proteases that cleave at phenylalanine sites.
• Structure-Activity Relationship (SAR) Studies: Employed to investigate the impact of D-amino acid incorporation on peptide bioactivity, stability, and receptor binding.
• Reference Standard: Serves as a high-purity analytical standard for quality control and method development in peptide analysis via HPLC or LC-MS.
• Bioconjugation & Labeling: Acts as a spacer or functional component in the design of peptide-drug conjugates, imaging probes, and diagnostic agents.

Basic Information

Product Name	H-Ala-D-Phe-Ala-Oh
CAS No.	155114-42-4
Molecular Formula	C17H23N3O5
Molecular Weight	349.38 g/mol
Synonyms	Ala-D-Phe-Ala; L-Alanyl-D-phenylalanyl-L-alanine; H-Ala-D-Phe-Ala-OH; Tripeptide Ala-D-Phe-Ala; A-D-F-A; D-Phenylalanine-containing tripeptide; (2S)-2-[[(2R)-2-amino-3-phenylpropanoyl]amino]propanoic acid; N-(L-Alanyl)-D-phenylalanyl-L-alanine
EINECS	Contact for details

Quality Control

Our H-Ala-D-Phe-Ala-Oh is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis to confirm the D-configuration, and identity confirmation by mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept under dry, inert conditions to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 97.0%
Chiral Purity (D-Phe)	≥ 99.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.