Description

Glycyl-7-Amino-4-Methylcoumarin-3-Acetic Acid is a specialized fluorogenic peptide derivative widely used as a key substrate in enzymatic assays and biochemical research. This compound is valued for its high sensitivity and specificity, enabling precise detection and quantification of protease activity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in drug discovery and proteomics.

Application

• Protease Activity Assays: Acts as a sensitive fluorogenic substrate for various proteolytic enzymes, including aminopeptidases and specific proteases.
• Biochemical Research: Used in kinetic studies and enzyme characterization to understand catalytic mechanisms and inhibition.
• Diagnostic Development: Serves as a critical component in the development of high-throughput screening (HTS) assays and clinical diagnostic kits.
• Pharmaceutical Screening: Employed in drug discovery pipelines to identify and evaluate potential protease inhibitors as therapeutic candidates.
• Cell Biology Studies: Utilized for detecting and imaging protease activity within live cells and tissue samples.

Basic Information

Product Name	Glycyl-7-Amino-4-Methylcoumarin-3-Acetic Acid
CAS No.	154653-66-4
Molecular Formula	C₁₅H₁₅N₃O₆
Molecular Weight	333.30 g/mol
Synonyms	Gly-AMC; Glycine 7-amido-4-methylcoumarin; Glycyl-AMCA; 7-(Glycylamino)-4-methylcoumarin-3-acetic Acid; AMC-Gly; Glycine-AMC derivative; Fluorogenic Glycine substrate; Peptidase substrate Gly-AMC
EINECS	Contact for details

Quality Control

Our Glycyl-7-Amino-4-Methylcoumarin-3-Acetic Acid is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for fine chemicals and biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to moisture and light. For long-term storage, consider desiccants and inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.