Description

n-(Alpha)Fluorenylmethoxycarbonyl-Glutamyl-Glutamyl-Phosphotyrosyl-Alanyl-Alanine is a specialized, high-purity synthetic peptide derivative, primarily used as a key intermediate in advanced pharmaceutical research and development. This compound is critical for studying and modulating protein-protein interactions, particularly those involving phosphotyrosine signaling pathways. It is essential for researchers and manufacturers in the fields of oncology, immunology, and signal transduction biochemistry, where precise molecular tools are required for drug discovery and biochemical assay development.

Application

• Pharmaceutical Intermediate: A critical building block for the synthesis of complex peptide-based drug candidates and active pharmaceutical ingredients (APIs).
• Biochemical Research: Used as a substrate, inhibitor, or standard in enzymatic assays to study kinase and phosphatase activity related to tyrosine phosphorylation.
• Drug Discovery: Serves as a key component in high-throughput screening (HTS) platforms to identify novel therapeutics targeting specific phosphotyrosine-binding domains (e.g., SH2 domains).
• Proteomics & Diagnostics: Employed in the development of affinity reagents, antibodies, and diagnostic kits for detecting phosphorylated proteins.
• Academic Research: A valuable tool for universities and research institutes studying cellular signaling, cancer biology, and metabolic disorders.
• Peptide Library Synthesis: Used in the generation of focused peptide libraries for structure-activity relationship (SAR) studies.

Basic Information

Product Name	n-(Alpha)Fluorenylmethoxycarbonyl-Glutamyl-Glutamyl-Phosphotyrosyl-Alanyl-Alanine
CAS No.	151957-36-7
Molecular Formula	C52H60N6O18P
Molecular Weight	1087.04 g/mol
Synonyms	Fmoc-Glu-Glu-pTyr-Ala-Ala-OH; Fmoc-E-E-pY-A-A-OH; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-glutamyl-L-glutamyl-O4-phosphono-L-tyrosyl-L-alanyl-L-alanine; Fmoc-Glu(OtBu)-Glu(OtBu)-Tyr(PO(OBzl)OH)-Ala-Ala-OH (protected form); Phosphotyrosine Peptide; pTyr-containing Peptide; Signal Transduction Peptide; SH2 Domain Ligand
EINECS	Contact for details

Quality Control

This high-value peptide is manufactured under strict cGMP-like conditions to ensure batch-to-batch consistency and traceability. Every lot is subjected to a comprehensive suite of analytical tests, including HPLC for purity, MS for identity confirmation, and amino acid analysis. Certificates of Analysis (COA) detailing all specifications and test results are provided with each shipment to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 90.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Single Impurity (HPLC)	≤ 2.0%
Peptide Content	≥ 70.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.