Description

Enkephalin, Pen(2,5)-Ala(3)- is a synthetic peptide analog of the endogenous opioid peptide enkephalin, specifically modified with penicillamine (Pen) and alanine (Ala) substitutions. This compound is of significant interest for its potential to modulate opioid receptor activity with enhanced metabolic stability and selectivity. It serves as a critical research-grade biochemical tool for neuroscientists and pharmacologists investigating pain pathways, neuropsychiatric disorders, and receptor-ligand interactions. The product is supplied under stringent quality control to ensure batch-to-batch consistency for reliable experimental results.

Application

• Neuroscience Research: Used as a selective ligand in studies of delta (δ) and mu (μ) opioid receptor binding, signaling, and distribution in the central nervous system.
• Pharmacology & Drug Discovery: Serves as a reference standard and lead compound in the development of novel, non-addictive analgesics and neurotherapeutics.
• Biochemical Assays: Employed in competitive binding assays, receptor autoradiography, and functional studies (e.g., cAMP accumulation, GTPγS binding) to characterize opioid system function.
• Metabolic Stability Studies: Acts as a model peptide to study the effects of D-amino acid (Pen) substitution on resistance to enzymatic degradation in vitro and in vivo.
• Academic & Institutional Research: A key reagent for university and government labs exploring the neurobiology of pain, addiction, and emotional regulation.

Basic Information

Product Name	Enkephalin, Pen(2,5)-Ala(3)-
CAS No.	151608-23-0
Molecular Formula	C34H48N6O8S2
Molecular Weight	732.91 g/mol
Synonyms	[D-Penicillamine2,5]-Enkephalin; [D-Pen2,5]-Enkephalin; DPDPE analog; Tyr-D-Pen-Gly-Phe-D-Pen-OH (with Ala substitution); Opioid peptide analog; δ-opioid receptor agonist; (2S)-2-amino-3-[[(2R)-2-amino-2-carboxyethyl]dithio]-N-[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]-3-methyl-1-oxobutan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]propanamide (IUPAC-like); H-Tyr-D-Pen-Gly-Phe-D-Pen-OH (Ala-modified)
EINECS	Contact for details

Quality Control

Every batch of Enkephalin, Pen(2,5)-Ala(3)- is manufactured and analyzed under a quality management system. Identity, purity, and composition are verified using advanced analytical techniques including HPLC and mass spectrometry to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, chromatographic data, and batch-specific information is provided with each shipment to guarantee traceability and reproducibility for your research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. The product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with theoretical molecular weight
Purity (HPLC)	≥ 95% (Area Percent)
Assay (Content)	≥ 90% (by weight)
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70% (by amino acid analysis)
Solubility	Soluble in water, dimethyl sulfoxide (DMSO)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.