Description

n-α-Fmoc-n-Im-Trityl-L-Histidine n-Carboxyanhydride is a highly specialized, protected amino acid derivative used as a key building block in solid-phase peptide synthesis (SPPS). This compound is critical for the controlled and efficient incorporation of histidine residues into peptide chains, particularly in the synthesis of complex peptides and proteins for research and therapeutic development. It is an essential reagent for pharmaceutical R&D, biotechnology companies, and academic laboratories focused on peptide-based drug discovery, vaccine development, and biochemical studies.

Application

• Solid-Phase Peptide Synthesis (SPPS): As an activated amino acid derivative (NCA) for the sequential, racemization-free coupling of Fmoc-protected histidine.
• Pharmaceutical Research: Synthesis of peptide active pharmaceutical ingredients (APIs) and investigational new drugs where histidine plays a structural or functional role.
• Bioconjugation & Labeling: Creating histidine-tagged peptides for purification, imaging, or diagnostic assay development.
• Vaccine Development: Incorporation into antigenic peptide sequences for epitope mapping and vaccine candidate production.
• Enzyme Mimic & Catalyst Research: Building peptides that mimic the active sites of metalloenzymes, often centered on histidine residues.
• High-Purity Reference Standard: Serves as a certified reference material for analytical method development and quality control in peptide manufacturing.

Basic Information

Product Name	n-α-Fmoc-n-Im-Trityl-L-Histidine n-Carboxyanhydride
CAS No.	151139-74-1
Molecular Formula	C46H37N3O5
Molecular Weight	711.81 g/mol
Synonyms	Fmoc-His(Trt)-NCA; Nα-Fmoc-Nim-trityl-L-histidine N-carboxyanhydride; (4S)-4-[(9H-Fluoren-9-ylmethoxy)carbonylamino]-5-[1-(triphenylmethyl)-1H-imidazol-4-yl]-1,3-oxazolidine-2,5-dione; Fmoc-L-histidine(Nim-Trt)-NCA; NCA-Fmoc-His(Trt)-OH; Fmoc-protected Histidine N-carboxyanhydride; Nα-(9-Fluorenylmethyloxycarbonyl)-Nim-trityl-L-histidine NCA
EINECS	Contact for details

Quality Control

Every batch of n-α-Fmoc-n-Im-Trityl-L-Histidine n-Carboxyanhydride is manufactured and tested under strict quality management systems. We ensure compliance with high-purity standards suitable for pharmaceutical research and peptide synthesis. Comprehensive analytical testing, including HPLC, NMR, and MS, is performed to verify identity, purity, and stability. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store in the original packaging.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines
Optical Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.