Description

Ac-Yvad-Amc is a fluorogenic peptide substrate specifically designed for the detection and quantification of caspase-1 (ICE) activity. This compound is a critical tool for researchers studying apoptosis and inflammatory pathways, providing high sensitivity and specificity in enzymatic assays. It is essential for pharmaceutical R&D, academic research, and diagnostic development focused on programmed cell death and immune response mechanisms.

Application

• Caspase-1 Activity Assays: Primary substrate for fluorometric and spectrophotometric determination of Interleukin-1β Converting Enzyme (ICE) activity in cell lysates and purified enzyme preparations.
• Apoptosis Research: Used to monitor caspase-1 activation during studies of pyroptosis and inflammatory cell death pathways.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel inhibitors or activators of caspase-1 for therapeutic development.
• Biochemical Research: Serves as a key reagent for kinetic studies, enzyme characterization, and mechanistic investigations of caspase family proteases.
• Diagnostic Development: Utilized in the development of assay kits for measuring caspase-1 activity as a biomarker in various disease models.

Basic Information

Product Name	Ac-Yvad-Amc
CAS No.	149231-65-2
Molecular Formula	C34H44N6O10
Molecular Weight	696.75 g/mol
Synonyms	Ac-Tyr-Val-Ala-Asp-AMC; Ac-YVAD-AMC; Ac-YVAD-7-amido-4-methylcoumarin; N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin; Caspase-1 Substrate IV; ICE Substrate; Fluorogenic Caspase-1 Substrate; Ac-YVAD-AFC (related); Ac-YVAD-pNA (related)
EINECS	Contact for details

Quality Control

Our Ac-Yvad-Amc is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and functional activity validation. Certificates of Analysis (COA) with detailed chromatographic data and assay results are provided to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms (Lot-specific data on COA)
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.