Description

Fmoc-o-Phospho-L-Tyrosine CAS NO 147762-53-6 is a protected, phosphorylated derivative of the amino acid L-tyrosine, specifically designed for solid-phase peptide synthesis (SPPS). This compound is a critical building block for the site-specific introduction of phosphotyrosine, enabling the study of protein phosphorylation—a fundamental regulatory mechanism in cell signaling. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and biochemical research who require high-purity, sequence-defined peptides to investigate kinase pathways, develop diagnostic tools, or create therapeutic candidates.

Application

• Solid-Phase Peptide Synthesis (SPPS): As an Fmoc-protected amino acid derivative for the automated synthesis of phosphopeptides.
• Phosphoproteomics Research: Synthesis of specific phosphotyrosine-containing peptide antigens for antibody production and immunoassay development.
• Kinase/Phosphatase Studies: Preparation of substrates and inhibitors to study enzyme activity, specificity, and signal transduction mechanisms.
• Drug Discovery: Building block for developing peptide-based therapeutics targeting phosphotyrosine-mediated protein-protein interactions (e.g., SH2 domains).
• Diagnostic Reagent Manufacturing: Production of standardized peptides for calibration and quality control in clinical diagnostic kits.
• Bioconjugation: Intermediate for creating phosphorylated protein conjugates or surface modifications for biosensor and microarray applications.

Basic Information

Product Name	Fmoc-o-Phospho-L-Tyrosine
CAS No.	147762-53-6
Molecular Formula	C24H22NO8P
Molecular Weight	483.41 g/mol
Synonyms	Fmoc-Tyr(PO3H2)-OH; N-(9-Fluorenylmethoxycarbonyl)-O-phosphono-L-tyrosine; Fmoc-O-phosphono-L-tyrosine; Fmoc-L-Tyrosine(PO3H2); Fmoc-Tyr(P)-OH; (2S)-3-(4-Phosphonooxyphenyl)-2-[(9H-fluoren-9-ylmethoxy)formamido]propanoic acid; Fmoc-P-Tyr-OH; Fmoc-Tyr[PO(OH)2]-OH
EINECS	Contact for details

Quality Control

Our Fmoc-o-Phospho-L-Tyrosine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and chiral analysis to ensure enantiomeric integrity. Certificates of Analysis (COA) with detailed chromatograms are provided, confirming compliance with in-house specifications tailored for peptide synthesis. We support cGMP manufacturing for advanced applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, seal under argon or nitrogen. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.