Description

Boc-[15N]Leu-Oh (CAS No. 146953-81-3) is a stable isotope-labeled derivative of the amino acid leucine, where the nitrogen atom is enriched with the 15N isotope and the α-amino group is protected by a tert-butoxycarbonyl (Boc) group. This compound is a critical building block for advanced research in proteomics, metabolic flux analysis, and the synthesis of isotopically labeled peptides and proteins. It is essential for scientists and manufacturers in the pharmaceutical, biotechnology, and academic research sectors who require high-purity, chemically defined tracers for mass spectrometry-based studies and the development of novel therapeutic agents.

Application

• Metabolic Pathway Tracing: Used as a stable isotope-labeled precursor in in vivo and in vitro studies to track protein synthesis, degradation, and amino acid metabolism using techniques like GC-MS or LC-MS.
• Quantitative Proteomics: Serves as a crucial reagent for the internal standard method (SILAC - Stable Isotope Labeling by Amino acids in Cell culture) to enable accurate protein quantification.
• Peptide & Protein Synthesis: Acts as a protected, isotopically labeled monomer in solid-phase and solution-phase peptide synthesis for producing labeled biomolecules for NMR and structural biology.
• Pharmaceutical Research: Employed in the research and development of new drugs, particularly in pharmacokinetic studies to understand drug metabolism and distribution.
• Biomarker Discovery: Facilitates the identification and validation of disease biomarkers by allowing precise measurement of protein turnover rates.
• Nutritional Science Research: Used in studies investigating amino acid utilization, protein requirements, and metabolic disorders.

Basic Information

Product Name	Boc-[15N]Leu-Oh
CAS No.	146953-81-3
Molecular Formula	C11H2115NO4
Molecular Weight	247.29 g/mol (for 15N isotopologue)
Synonyms	N-(tert-Butoxycarbonyl)-L-leucine-15N; Boc-L-leucine-15N; (2S)-2-[(tert-Butoxycarbonyl)(15N)amino]-4-methylpentanoic acid; N-Boc-L-leucine-15N; tert-Butyloxycarbonyl-15N-leucine; Boc-Leu-15N-OH; L-Leucine-15N, N-BOC protected
EINECS	Contact for details

Quality Control

Every batch of Boc-[15N]Leu-Oh is manufactured and tested under strict quality management systems to ensure the highest standards of isotopic and chemical purity, which are critical for sensitive analytical applications. Our quality control protocols include comprehensive analytical techniques to verify identity, isotopic enrichment, chiral purity, and the absence of critical impurities. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting key parameters such as assay (HPLC), isotopic enrichment (NMR/MS), optical rotation, and residual solvent content.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, consider sealing under argon or nitrogen. Keep the container tightly sealed in a desiccator to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Isotopic Enrichment (15N)	≥98 atom % 15N
Optical Rotation [α]D20	-20° to -24° (c=1 in Acetic Acid)
Chiral Purity (HPLC)	≥99.0% (L-isomer)
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.