Description

n-[4-[2-(2-Amino-4,7-Dihydro-4-Oxo-3H-Pyrrolo[2,3-D]Pyrimidin-5-Yl)Ethyl]Benzoyl]-L-Glutamic Acid 1,5-Diethyl Ester is a high-purity, non-classical folate analog intermediate of significant importance in advanced pharmaceutical research and development. This compound matters as a critical building block for the synthesis of novel antifolate agents, which are pivotal in targeting specific metabolic pathways. It is primarily needed by pharmaceutical R&D laboratories, contract research organizations (CROs), and fine chemical manufacturers focused on oncology, autoimmune diseases, and other therapeutic areas involving folate metabolism.

Application

• Pharmaceutical Intermediate: Key precursor in the synthesis of novel antifolate drugs and targeted therapies.
• Oncology Research: Used in the development of chemotherapeutic agents that inhibit dihydrofolate reductase (DHFR) and other folate-dependent enzymes.
• Biochemical Research: Serves as a tool compound for studying folate metabolism, cellular uptake mechanisms, and enzyme inhibition kinetics.
• Contract Manufacturing: Supplied to CDMOs (Contract Development and Manufacturing Organizations) for scale-up and GMP production of active pharmaceutical ingredients (APIs).
• Academic Research: Utilized in university and institutional labs for medicinal chemistry projects and drug discovery programs.
• Reference Standard: Can be qualified for use as an analytical reference standard in quality control laboratories.

Basic Information

Product Name	n-[4-[2-(2-Amino-4,7-Dihydro-4-Oxo-3H-Pyrrolo[2,3-D]Pyrimidin-5-Yl)Ethyl]Benzoyl]-L-Glutamic Acid 1,5-Diethyl Ester
CAS No.	146943-43-3
Molecular Formula	C24H27N5O6
Molecular Weight	481.50 g/mol
Synonyms	L-Glutamic acid, N-[4-[2-(2-amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-, 1,5-diethyl ester; 5,10-Dideazapteroylglutamate diethyl ester; DDATHF Diethyl Ester; (6R)-N-[4-[[(2-Amino-3,4-dihydro-4-oxo-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]glutamic acid diethyl ester; Lometrexol Diethyl Ester Intermediate; Non-classical folate analog intermediate; Antifolate intermediate.
EINECS	Contact for details

Quality Control

Our n-[4-[2-(2-Amino-4,7-Dihydro-4-Oxo-3H-Pyrrolo[2,3-D]Pyrimidin-5-Yl)Ethyl]Benzoyl]-L-Glutamic Acid 1,5-Diethyl Ester is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to meet specific project requirements. We support development with research-grade to GMP-grade material, ensuring supply chain reliability for critical R&D and pre-clinical phases.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation or storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Single Unknown Impurity (HPLC)	≤ 1.0%
Total Impurities (HPLC)	≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.