Description

n-Dansylmethionyl-Leucyl-Phenylalanine is a synthetic, dansyl-labeled tripeptide derivative designed for advanced biochemical research. This compound matters as a highly specific and sensitive fluorescent probe, enabling the study of protease activity and enzyme kinetics. It is primarily needed by researchers in pharmaceutical development, biochemistry, and proteomics for applications in assay development and molecular diagnostics.

Application

• Fluorescent Substrate for Protease Assays: Used as a specific, sensitive substrate to measure the activity of enzymes like chymotrypsin and related serine proteases.
• Enzyme Kinetics Studies: Enables real-time monitoring of enzymatic cleavage rates and inhibition studies due to its dansyl fluorophore.
• High-Throughput Screening (HTS): Serves as a key reagent in drug discovery platforms to identify and characterize novel protease inhibitors.
• Biochemical Research Tool: Employed in academic and industrial labs to investigate protein-protein interactions and signal transduction pathways involving proteolytic cleavage.
• Diagnostic Assay Development: Acts as a core component in developing fluorescent diagnostic kits for detecting specific protease biomarkers.

Basic Information

Product Name	n-Dansylmethionyl-Leucyl-Phenylalanine
CAS No.	146935-91-3
Molecular Formula	C33H44N4O7S2
Molecular Weight	672.85 g/mol
Synonyms	Dansyl-Met-Leu-Phe; Dns-Met-Leu-Phe; N-Dansyl-Methionyl-Leucyl-Phenylalanine; N-Dansyl-MLF; Fluorescent Peptide Substrate MLF; 5-(Dimethylamino)naphthalene-1-sulfonyl-Met-Leu-Phe; Protease Substrate III (Chymotrypsin); L-Methionyl-L-leucyl-L-phenylalanine, 1-[5-(dimethylamino)-1-naphthalenesulfonyl]-
EINECS	Contact for details

Quality Control

Our n-Dansylmethionyl-Leucyl-Phenylalanine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, MS for identity confirmation, and NMR for structural verification, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance. Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.