Description

Alanyl-Threonyl-Phosphotyrosyl-Seryl-Alanine is a synthetic, phosphorylated pentapeptide sequence of significant interest in biochemical and pharmaceutical research. This compound serves as a critical tool for studying signal transduction pathways, specifically those involving tyrosine phosphorylation and protein-protein interactions. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in kinase/phosphatase assay development, antibody production, and targeted drug discovery programs.

Application

• Biochemical Research Reagent: Used as a substrate or inhibitor in enzymatic assays to study the activity of protein tyrosine kinases (PTKs) and phosphatases (PTPs).
• Antigen for Antibody Production: Serves as a hapten or immunogen for generating and screening phospho-specific antibodies.
• Cell Signaling Pathway Analysis: Employed in competitive binding studies and pull-down assays to investigate intracellular signaling mechanisms.
• Diagnostic Kit Development: Incorporated as a key component in ELISA and other immunoassay kits for detecting phosphorylation-related diseases.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and validate novel kinase-targeted therapeutic candidates.
• Peptide Synthesis Standard: Acts as a reference standard for quality control in custom peptide synthesis and analytical method validation.

Basic Information

Product Name	Alanyl-Threonyl-Phosphotyrosyl-Seryl-Alanine
CAS No.	145079-49-8
Molecular Formula	C23H34N5O13P
Molecular Weight	619.52 g/mol
Synonyms	ATpYSA; H-Ala-Thr-pTyr-Ser-Ala-OH; Phosphotyrosine-containing Pentapeptide; pTyr Peptide Substrate; L-Alanyl-L-threonyl-O-phosphono-L-tyrosyl-L-seryl-L-alanine; Kinase Substrate Peptide; Signal Transduction Peptide; Research Peptide 145079-49-8
EINECS	Contact for details

Quality Control

Every batch of Alanyl-Threonyl-Phosphotyrosyl-Seryl-Alanine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our standard quality commitment includes comprehensive characterization by HPLC, MS, and NMR. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Single Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.