Description

Alanyl-Phosphothreonyl-Phosphotyrosyl-Seryl-Alanine is a synthetic phosphopeptide sequence, a crucial tool compound for biochemical and pharmaceutical research. This compound is specifically designed to mimic phosphorylated protein motifs, enabling the study of signal transduction pathways, kinase-substrate interactions, and antibody development. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in proteomics, assay development, and targeted drug discovery programs.

Application

• Biochemical Research: As a specific substrate or inhibitor in enzymatic assays to study protein kinases, phosphatases, and their regulatory mechanisms.
• Antibody Production & Validation: Serves as a key immunogen and screening antigen for generating and characterizing phospho-specific antibodies.
• Diagnostic Assay Development: Used as a calibrated standard or capture molecule in ELISA and other immunoassays for detecting protein phosphorylation states.
• Cell Signaling Studies: A tool for investigating intracellular signaling cascades related to the specific phosphorylated sequence it represents.
• Mass Spectrometry Standard: Acts as a reference standard in proteomic analysis for method development and quantification of phosphorylated peptides.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify potential therapeutic compounds that modulate specific phosphorylation events.

Basic Information

Product Name	Alanyl-Phosphothreonyl-Phosphotyrosyl-Seryl-Alanine
CAS No.	145079-47-6
Molecular Formula	C23H34N5O16P2
Molecular Weight	722.49 g/mol
Synonyms	ATPYS Peptide; pThr-pTyr Peptide; Phospho-Threonine-Tyrosine Peptide; A-pT-pY-S-A; H-Ala-pThr-pTyr-Ser-Ala-OH; Phosphopeptide A(pT)(pY)SA; Alanyl-O-phosphonothreonyl-O-phosphonotyrosyl-seryl-alanine
EINECS	Contact for details

Quality Control

Every batch of our Alanyl-Phosphothreonyl-Phosphotyrosyl-Seryl-Alanine is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We guarantee high purity and batch-to-batch consistency through rigorous testing via HPLC, MS, and NMR. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment to ensure compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80%
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.