Description

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans CAS NO 142988-22-5 is a specialized, high-purity peptide substrate designed for sensitive biochemical research and diagnostic applications. This compound serves as a critical tool for studying enzyme kinetics, particularly for proteases like renin and other aspartic proteases, due to its intramolecularly quenched fluorescent (IQF) design featuring Dabcyl and Edans fluorophores. It is essential for researchers and developers in pharmaceutical discovery, clinical diagnostics, and advanced life science research who require reliable, high-quality substrates for assay development and high-throughput screening.

Application

• Protease Activity Assays: Primary substrate for continuous fluorometric assays targeting renin and related aspartic proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors.
• Enzyme Kinetic Studies: Enables precise measurement of kinetic parameters (Km, Vmax, kcat) for detailed mechanistic studies.
• Diagnostic Kit Development: A key component in the development of in vitro diagnostic (IVD) assays for conditions related to protease activity.
• Biochemical Research: Tool for investigating the role of specific proteolytic cleavage events in cellular pathways and disease models.
• Quality Control in Biopharma: Used to monitor the activity and specificity of enzyme preparations during manufacturing processes.

Basic Information

Product Name	Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans
CAS No.	142988-22-5
Molecular Formula	C86H118N22O19S2
Molecular Weight	1764.14 g/mol
Synonyms	Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans; Renin Substrate, Fluorogenic; 4-((4-(Dimethylamino)phenyl)azo)benzoic Acid-γ-Aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS; Intramolecularly Quenched Fluorescent Peptide; IQF Renin Substrate; Dabcyl-EDANS FRET Peptide; FRET-based Protease Substrate; (EDANS)-GABA-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-(DABCYL)
EINECS	Contact for details

Quality Control

Every batch of this peptide substrate is manufactured under strict quality control protocols to ensure consistency and performance for critical research applications. Our standard grade meets high-purity specifications suitable for biochemical assays, with identity and purity verified by advanced analytical techniques. Certificates of Analysis (COA) detailing purity, HPLC profile, and mass spectrometry data are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass consistent with theoretical molecular weight
Purity (HPLC)	≥ 95% (Area Percent)
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.