Description

3-(2'-(Spiro-5-Chloroadamantane))-4-Methoxy-4-(3''-Phosphoryloxy)Phenyl-1,2-Dioxetane CAS NO 142456-88-0 is a highly specialized, chemiluminescent dioxetane derivative designed for advanced analytical applications. Its core value lies in its ability to serve as a highly sensitive and stable substrate for enzymatic detection systems, particularly in immunoassays and nucleic acid hybridization assays. This compound is essential for manufacturers and research institutions in the in-vitro diagnostics (IVD), life science research, and pharmaceutical development sectors, where precise and low-background signal generation is critical.

Application

• Chemiluminescent Substrate for Alkaline Phosphatase (AP): Primary application as a high-sensitivity, stable light-emitting substrate in enzyme-linked immunosorbent assays (ELISAs), Western blots, and Southern/Northern blots.
• Clinical Diagnostics: Used in automated immunoassay systems for the detection of hormones, tumor markers, infectious diseases, and cardiac markers.
• Molecular Biology: Key component in non-radioactive nucleic acid detection kits for DNA sequencing, in-situ hybridization, and microarray analysis.
• Biosensor Development: Integrated into sensor platforms for environmental monitoring, food pathogen detection, and point-of-care testing devices.
• Research Reagents: Supplied as a critical raw material for life science companies formulating their own ultra-sensitive detection kits.
• Forensic Analysis: Employed in kits requiring high sensitivity for low-abundance sample analysis.

Basic Information

Product Name	3-(2'-(Spiro-5-Chloroadamantane))-4-Methoxy-4-(3''-Phosphoryloxy)Phenyl-1,2-Dioxetane
CAS No.	142456-88-0
Molecular Formula	C23H28ClO7P
Molecular Weight	482.89 g/mol
Synonyms	5-Chloro-3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo[3.3.1.13,7]decan]-4-yl)phenyl phosphate; Disodium 4-Methoxy-4-(3-phosphoryloxyphenyl)spiro(1,2-dioxetane-3,2'-adamantane); CSPD® (Registered trademark substrate); Chemiluminescent Alkaline Phosphatase Substrate; 1,2-Dioxetane, 3-(2-(5-chloroadamantyl))-4-methoxy-4-(3-phosphoryloxyphenyl)-; AP Chemiluminescent Substrate; Lumigen APS-5; 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxyphenyl)-1,2-dioxetane, 5'-chloro derivative
EINECS	Contact for details

Quality Control

Every batch of our chemiluminescent substrate is manufactured under strict quality management systems and undergoes rigorous analytical testing to ensure exceptional performance and lot-to-lot consistency. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and functional performance in standard assay systems. Our production adheres to principles of GMP for active pharmaceutical ingredients (APIs) and is designed to meet the stringent requirements of the in-vitro diagnostics (IVD) industry.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is easily oxidized; for long-term storage, containers should be sealed under an inert atmosphere such as argon or nitrogen. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH Q3C guidelines
Functional Activity	≥ 95% of reference standard in chemiluminescent assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.