Description

Spt5 Protein CAS NO 138673-72-0 is a highly purified recombinant protein, specifically the Spt5 subunit of the DSIF (DRB sensitivity-inducing factor) complex, which plays a critical role in the regulation of RNA polymerase II transcription. This protein is essential for fundamental research into transcriptional pausing, elongation, and co-transcriptional processes. It is primarily utilized by researchers and developers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on gene expression, epigenetics, and novel therapeutic target discovery.

Application

• Biochemical Research: Used in in vitro transcription assays to study the mechanism of transcriptional pausing and elongation.
• Protein-Protein Interaction Studies: Essential for investigating the assembly and function of the DSIF and P-TEFb complexes.
• Drug Discovery Screening: Serves as a target protein in high-throughput screens for identifying potential inhibitors of transcription for antiviral or anticancer applications.
• Enzymology: Employed in studies to understand the kinase activity of P-TEFb on the RNA polymerase II C-terminal domain (CTD).
• Structural Biology: Used for X-ray crystallography, cryo-electron microscopy, and NMR studies to determine the 3D structure of transcription complexes.
• Diagnostic Development: Can be used as a component in assays designed to detect or quantify specific transcriptional regulators or their activities.

Basic Information

Product Name	Spt5 Protein
CAS No.	138673-72-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	SUPT5H Protein; Transcription elongation factor SPT5; DSIF p160 subunit; FACT p160 homolog; CDC73 homolog; Spt5; SUPT5H; KOW; Transcription elongation regulator 1
EINECS	Contact for details

Quality Control

Our Spt5 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional activity assays, and endotoxin level verification. Certificates of Analysis (COA) detailing identity, purity, concentration, and biological activity are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle with appropriate laboratory precautions.

Specification

Item	Specification
Appearance	Clear or slightly opalescent solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (HPLC)	≥ 90%
Protein Concentration	As per COA (typically 0.1 - 1.0 mg/mL)
Biological Activity	Functional in in vitro transcription assay
Endotoxin Level	< 1.0 EU/µg
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.