Description

Big Endothelin-1 (1-39) (Rat) is a synthetic peptide fragment corresponding to the full-length, 39-amino acid precursor of the potent vasoconstrictor Endothelin-1. This compound is a critical research tool for studying the biosynthesis, processing, and biological activity of endothelin peptides in rodent models. It is primarily utilized by researchers in pharmacology, cardiovascular biology, and molecular biology for in vitro and in vivo investigations.

Application

• Biochemical Research: Used as a substrate to study the enzymatic conversion of Big Endothelin-1 to mature Endothelin-1 by endothelin-converting enzymes (ECEs).
• Vascular Biology Studies: Essential for investigating the precursor-product relationship and the regulatory mechanisms of endothelin synthesis in rat-based experimental systems.
• Receptor Binding Assays: Serves as a key ligand in competitive binding studies to characterize endothelin receptor subtypes (ETA and ETB).
• Antibody Production & Immunoassays: Acts as an immunogen for generating specific antibodies or as a standard in ELISA and RIA for detecting Big Endothelin-1.
• Cell Signaling Pathway Analysis: Employed to stimulate cells and analyze downstream signaling cascades activated by endothelin precursor peptides.
• Pharmaceutical Screening: Used in high-throughput screens to identify and evaluate potential inhibitors of endothelin-converting enzymes for drug discovery.

Basic Information

Product Name	Big Endothelin-1 (1-39) (Rat)
CAS No.	135842-15-8
Molecular Formula	C198H302N56O57S5
Molecular Weight	~4430.9 g/mol
Synonyms	Big ET-1 (1-39), rat; Endothelin-1 (1-39), rat; Preproendothelin-1 (1-39) (rat); ET-1 (1-39); Big Endothelin; Porcine Big Endothelin-1 (commonly referenced); Human Big Endothelin-1 (1-38) (related isoform); ppET-1 (1-39)
EINECS	Contact for details

Quality Control

Our Big Endothelin-1 (1-39) (Rat) is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency for research applications. Each lot undergoes comprehensive analytical characterization. Certificates of Analysis (COA) are provided, detailing purity, identity, and peptide content as determined by HPLC, mass spectrometry, and amino acid analysis.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.