Description

Biocytin-β-Endorphin Human is a synthetic, biotinylated peptide analog of the endogenous human β-endorphin hormone. This compound is engineered to facilitate high-affinity binding and detection in advanced biochemical research and diagnostic applications. It is primarily utilized by researchers and developers in the fields of neuroscience, pharmacology, and molecular diagnostics for studying opioid receptor interactions, signal transduction pathways, and developing novel assay systems.

Application

• Neuroscience Research: As a tagged ligand for the study of opioid receptor (MOR, DOR, KOR) localization, binding affinity, and internalization dynamics.
• Diagnostic Assay Development: Serving as a critical capture or detection reagent in ELISA, Western blot, and other immunoassay formats for β-endorphin quantification.
• Affinity Purification & Pull-Down Assays: Leveraging the biotin tag for streptavidin-based isolation of receptor complexes and interacting proteins.
• Cellular Imaging: Used in fluorescence microscopy and flow cytometry when conjugated to fluorophore-labeled streptavidin for visualizing receptor distribution.
• Pain & Addiction Research: A key tool for investigating the mechanisms of analgesia, stress response, and the neurobiology of substance use disorders.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel agonists or antagonists targeting the opioid receptor pathway.

Basic Information

Product Name	Biocytin-β-Endorphin Human
CAS No.	135482-75-6
Molecular Formula	C150H246N42O46S
Molecular Weight	3352.9 g/mol (approximately)
Synonyms	β-Endorphin (human), biotinylated; Biotinyl-β-Endorphin; Biotinylated β-Endorphin; N-Biotinyl-β-endorphin; H-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys-Asn-Ala-Ile-Ile-Lys-Asn-Ala-Tyr-Lys-Lys-Gly-Glu-Lys-Biotin; Biocytin-β-Endorphin; Biotin-β-Endorphin
EINECS	Contact for details

Quality Control

Our Biocytin-β-Endorphin Human is manufactured under strict quality-controlled conditions suitable for research use. Each batch is characterized to ensure high purity and consistent biological activity. Identity is confirmed by mass spectrometry (MS) and HPLC, while purity is assessed via analytical HPLC. Certificates of Analysis (COA) detailing purity, peptide content, and analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use vials to minimize freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, dilute acetic acid, or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.