Description

Insulin, His(B16)- is a specifically engineered insulin analog where the amino acid at position B16 has been substituted with a histidine residue. This targeted modification is of significant research interest for studying the structure-activity relationships of insulin and its interaction with the insulin receptor. This high-purity biochemical is essential for advanced pharmaceutical research, particularly in the fields of diabetes, metabolic disorder studies, and peptide hormone engineering.

Application

• Pharmaceutical Research & Development: A critical reference standard and intermediate for investigating insulin's mechanism of action, receptor binding affinity, and metabolic effects.
• Structure-Activity Relationship (SAR) Studies: Used to elucidate the impact of specific amino acid substitutions on insulin's potency, stability, and pharmacokinetic profile.
• Biochemical Assay Development: Serves as a key component in developing and validating assays for insulin detection, quantification, and bioactivity testing.
• Academic & Clinical Research: Supports fundamental research in endocrinology, diabetes mellitus, and peptide-based therapeutics.
• Peptide Chemistry & Engineering: Acts as a model compound for studying peptide folding, stability, and the development of novel insulin analogs with improved properties.

Basic Information

Product Name	Insulin, His(B16)-
CAS No.	135059-20-0
Molecular Formula	C257H383N65O77S6
Molecular Weight	5807.9 g/mol (approx.)
Synonyms	B16-His Insulin; [HisB16]-Insulin; Insulin (human) B16-His analog; Histidine(B16)-Insulin; Insulin analog (B16→His); Insulin, His16-B-chain; Recombinant Human Insulin His(B16) variant
EINECS	Contact for details

Quality Control

Our Insulin, His(B16)- is manufactured under stringent conditions to ensure the highest standards of purity and consistency, suitable for sensitive research applications. Each batch is subjected to a comprehensive battery of analytical tests, including HPLC for purity, mass spectrometry for identity confirmation, and peptide content analysis. A detailed Certificate of Analysis (COA) is provided, documenting all critical quality attributes to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Peptide Content (UV)	≥ 80.0%
Water Content (KF)	≤ 5.0%
Acetic Acid Content (HPLC)	≤ 10.0%
Biological Activity	Assessed by receptor binding assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.