Description

Big Endothelin-1 (1-31) (Human, Bovine) CAS NO 133972-52-8 is a synthetic peptide fragment corresponding to the N-terminal 1-31 amino acid sequence of the potent vasoconstrictor, Endothelin-1. This compound is a critical research tool for investigating the structure-activity relationships and signaling pathways of endothelin peptides. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and biotechnology companies focused on cardiovascular biology, hypertension research, and the development of novel therapeutic agents targeting the endothelin system.

Application

• Biochemical Research: Used as a reference standard and active agent in studies of endothelin receptor binding, activation, and downstream signaling cascades.
• Cardiovascular Pharmacology: Essential for in vitro and ex vivo assays to study vasoconstriction, vascular smooth muscle cell proliferation, and the pathophysiology of hypertension and heart failure.
• Drug Discovery & Development: Serves as a key ligand for screening and characterizing potential endothelin receptor antagonists and modulators in high-throughput screening (HTS) platforms.
• Academic Studies: Employed in university and institutional labs to explore the role of endothelin in various physiological and disease models beyond the cardiovascular system.
• Peptide Chemistry Reference: Acts as a standard for analytical method development (HPLC, MS) and for quality control of related synthetic peptide batches.

Basic Information

Product Name	Big Endothelin-1 (1-31) (Human, Bovine)
CAS No.	133972-52-8
Molecular Formula	C149H244N44O47S5
Molecular Weight	3485.1 g/mol
Synonyms	Endothelin-1 (1-31), human, porcine; Big ET-1 (1-31); ET-1 (1-31); Preproendothelin-1 (1-31); [Cys1,15, Ala3,11, Leu7, Asp8, Lys9, Glu10, Tyr13,14, Phe22, Trp31]-Endothelin-1; H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Ile-Asp-Pro-Ile-His-Leu-Val-His-Pro-Met-OH (Disulfide bridge: Cys1-Cys15)
EINECS	Contact for details

Quality Control

Our Big Endothelin-1 (1-31) is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. All products undergo rigorous quality testing, including reversed-phase HPLC and mass spectrometry (MS) for identity and purity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, peptide content, and analytical data is provided with each batch to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Molecular weight confirmed by mass spectrometry
Purity (HPLC)	≥ 95% (Area Percent)
Peptide Content	≥ 70% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical composition
Solubility	Soluble in water or aqueous acetic acid

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.