Description

Cbf1 Protein CAS NO 133837-28-2 is a highly purified recombinant protein, specifically the centromere-binding factor 1, which plays a critical role in chromosomal segregation and kinetochore assembly. This protein is essential for researchers and developers in the life sciences sector requiring high-quality, biologically active components for molecular and cellular biology studies. It is primarily utilized in biochemical research, diagnostic assay development, and as a key reagent in the study of cell division mechanisms and genetic stability.

Application

• Biochemical Research: Used as a critical reagent in studies of chromosome biology, kinetochore function, and cell cycle regulation.
• Diagnostic Development: Serves as a reference standard or capture molecule in the development of assays targeting cell division biomarkers.
• Enzymology Studies: Employed in investigations of DNA-protein interactions and the mechanistic study of centromere binding.
• Pharmaceutical R&D: Utilized in target validation and screening platforms for novel therapeutics aimed at cell cycle dysregulation, such as in oncology.
• Academic Research: A fundamental tool for university and institutional labs exploring mitosis, meiosis, and genomic integrity.
• Reagent Production: Acts as a starting material or standard for the production of specialized kits and research-grade biochemicals.

Basic Information

Product Name	Cbf1 Protein
CAS No.	133837-28-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CBF1; Centromere-binding factor 1; Cbf1p; CPF1; Yeast Cbf1 protein; Recombinant Cbf1 Protein; Centromere protein CBF1; Kinetochore protein Cbf1
EINECS	Contact for details

Quality Control

Our Cbf1 Protein is manufactured under stringent quality systems. Each batch undergoes rigorous analytical testing, including purity assessment by SDS-PAGE and HPLC, activity assays, and endotoxin level checks to ensure it meets high research-grade standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity, biological activity, and storage conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product should be handled in accordance with good laboratory practices for biological materials.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Concentration	As per COA (typically 0.1 - 1.0 mg/mL)
Biological Activity	Confirmed by functional assay (e.g., DNA-binding EMSA)
Endotoxin Level	< 1.0 EU/µg
Buffer Composition	Tris or Phosphate buffer, pH 7.0-7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.