Description

Big Endothelin-3 (1-41) Amide (Human) Trifluoroacetate Salt is a high-purity synthetic peptide corresponding to the full-length, biologically active form of human endothelin-3. This compound is a critical research tool for studying the endothelin system, which plays a vital role in cardiovascular regulation, cell proliferation, and neural development. It is essential for scientists and pharmaceutical developers in academic research, biotechnology, and drug discovery programs focused on vasoconstriction, cancer biology, and neurological disorders.

Application

• Biomedical Research: Used as a standard or agonist in receptor binding assays, signal transduction studies, and functional characterization of endothelin receptor subtypes (ETA and ETB).
• Drug Discovery & Development: Serves as a key pharmacological tool for screening and validating potential endothelin receptor antagonists in cardiovascular and oncology therapeutic areas.
• Cell Biology Studies: Applied in vitro to investigate the effects of endothelin-3 on cell proliferation, migration, and differentiation in various cell lines.
• Neuroscience Research: Utilized to study the role of endothelin-3 in the enteric nervous system development and its implications in Hirschsprung's disease.
• Diagnostic Development: May be employed in the development of immunoassays (ELISA, RIA) for the detection and quantification of endothelin-3 or related antibodies.
• Peptide Chemistry Reference: Acts as a high-purity reference standard for analytical method development, quality control, and mass spectrometry.

Basic Information

Product Name	Big Endothelin-3 (1-41) Amide (Human) Trifluoroacetate Salt
CAS No.	133551-97-0
Molecular Formula	C212H327N57O61S5 • xC2HF3O2
Molecular Weight	~4632.6 g/mol (free peptide base)
Synonyms	Endothelin-3 (1-41) amide (human); ET-3 (1-41); Big ET-3; Endothelin-3 precursor (1-41); hET-3(1-41)-NH2; Preproendothelin-3 (1-41) amide; Trifluoroacetic acid salt of Endothelin-3 (1-41) amide; Big Endothelin-3
EINECS	Contact for details

Quality Control

Our Big Endothelin-3 (1-41) Amide is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and peptide content. We adhere to rigorous quality testing protocols, and specifications can be aligned with customer requirements for advanced research or development purposes.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep under inert atmosphere or in a vacuum-sealed container. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with theoretical molecular weight
Purity (HPLC)	≥ 95% (Area % at 214 nm)
Peptide Content	≥ 70% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical composition
Water Content (KF)	≤ 8.0%
Trifluoroacetate Content	≤ 15.0% (by Ion Chromatography)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.