Description

Fmoc-o-Trityl-L-Threonine CAS NO 133180-01-5 is a high-purity, orthogonally protected derivative of the amino acid L-threonine, specifically engineered for solid-phase peptide synthesis (SPPS). This compound is critical for researchers and manufacturers requiring precise control over side-chain reactivity during the sequential assembly of complex peptides. It is an essential building block for pharmaceutical R&D, particularly in the development of therapeutic peptides, vaccines, and diagnostic agents.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the Fmoc/tBu strategy, allowing for the selective deprotection of the α-amino group while the side-chain hydroxyl remains shielded by the acid-labile trityl group.
• Pharmaceutical Intermediate: Serves as a crucial raw material in the synthesis of peptide-based active pharmaceutical ingredients (APIs) for therapeutic applications.
• Research & Development: Used in academic and industrial laboratories for the discovery and development of novel peptide structures, including hormones, antibiotics, and enzyme inhibitors.
• Combinatorial Chemistry: Employed in the generation of peptide libraries for high-throughput screening in drug discovery programs.
• Bioconjugation: The protected threonine residue can be incorporated into peptides destined for conjugation with other molecules, such as dyes, polymers, or proteins.
• Glycopeptide Synthesis: The O-trityl protection is a common strategy for the synthesis of O-linked glycopeptides, where the hydroxyl group of threonine serves as an attachment point for carbohydrates.

Basic Information

Product Name	Fmoc-o-Trityl-L-Threonine
CAS No.	133180-01-5
Molecular Formula	C43H37NO5
Molecular Weight	647.76 g/mol
Synonyms	Fmoc-Thr(Trityl)-OH; N-(9-Fluorenylmethoxycarbonyl)-O-trityl-L-threonine; Fmoc-L-Threonine(O-trityl); (2S,3R)-3-[(Trityloxy)-2-[(9H-fluoren-9-ylmethoxy)carbonyl]amino]butanoic acid; N-α-Fmoc-O-trityl-L-threonine; Fmoc-Thr(Trt)-OH; Fmoc-O-Trt-L-Thr-OH; Fmoc-L-Thr(Trt)-OH
EINECS	Contact for details

Quality Control

Our Fmoc-o-Trityl-L-Threonine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, essential for sensitive peptide synthesis applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and chiral integrity, and is supplied with a corresponding Certificate of Analysis (COA). We adhere to cGMP principles for pharmaceutical intermediates, and specifications can be tailored to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥98.0%
Optical Rotation	[α]²⁰D = +15.0° to +20.0° (c=1 in DMF)
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.