Description

1-[2-(2-Aminoethoxy)Ethyl]Maleimide-Hcl is a specialized bifunctional linker molecule featuring both a maleimide group and a primary amine, separated by a flexible polyether spacer. This compound is critical for creating stable, site-specific conjugates in bioconjugation chemistry, particularly for attaching biomolecules like antibodies, peptides, or proteins to various surfaces, drugs, or detection probes. It is primarily utilized by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for developing advanced therapeutics and assay systems.

Application

• Bioconjugation Reagent: For the site-specific covalent coupling of thiol-containing biomolecules (e.g., antibodies, proteins, peptides) to amine-functionalized surfaces, particles, or other molecules.
• Antibody-Drug Conjugate (ADC) Development: Serves as a heterobifunctional crosslinker in the synthesis of novel ADC payloads, connecting cytotoxic drugs to monoclonal antibodies.
• Diagnostic Assay Fabrication: Used to immobilize capture proteins or antibodies onto sensor chips, microplates, or nanoparticles for ELISA, lateral flow assays, and biosensors.
• Polymer and Material Science: Acts as a functional monomer or crosslinking agent for synthesizing smart polymers, hydrogels, and functionalized nanomaterials with reactive handles.
• Peptide and Protein Labeling: Enables the introduction of fluorescent dyes, affinity tags (e.g., biotin), or other probes onto cysteine residues within protein structures.
• Research Tool in Chemical Biology: Employed in studying protein-protein interactions, cell surface labeling, and the construction of complex biomolecular assemblies.

Basic Information

Product Name	1-[2-(2-Aminoethoxy)Ethyl]Maleimide-Hcl
CAS No.	131274-16-3
Molecular Formula	C10H15ClN2O4
Molecular Weight	262.69 g/mol
Synonyms	N-(2-(2-Aminoethoxy)ethyl)maleimide hydrochloride; 2,5-Pyrrolidinedione, 1-[2-[2-aminoethoxy]ethyl]-, hydrochloride (1:1); Maleimide, N-[2-(2-aminoethoxy)ethyl]-, hydrochloride; Amino-PEG2-maleimide hydrochloride; AM-PEG2-Mal HCl; 1-[2-(2-Aminoethoxy)ethyl]-1H-pyrrole-2,5-dione hydrochloride; Heterobifunctional PEG crosslinker (amine and maleimide)
EINECS	Contact for details

Quality Control

Our 1-[2-(2-Aminoethoxy)Ethyl]Maleimide-Hcl is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure it meets high-purity standards suitable for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.