Description

Amaninamide, S-Deoxo-Abu(1)-Ile(3)- is a structurally defined cyclic octapeptide analog derived from α-amanitin, engineered to retain high-affinity binding to RNA polymerase II while modulating cytotoxicity and metabolic stability. This compound serves as a critical pharmacological tool for studying transcriptional inhibition mechanisms and developing next-generation targeted therapeutics in oncology research. It is primarily utilized by biopharmaceutical R&D laboratories, academic research institutions, and contract development and manufacturing organizations (CDMOs) engaged in novel anticancer agent discovery and mechanism-of-action validation.

Application

• Targeted inhibition of RNA polymerase II in mechanistic studies of transcriptional regulation
• Lead compound optimization in structure–activity relationship (SAR) programs for amatoxin-based antitumor agents
• Cell-based assay development for high-throughput screening of transcriptional inhibitors
• Conjugation platform for antibody–drug conjugates (ADCs) targeting proliferating cancer cells
• Reference standard in LC-MS/MS quantification methods for amatoxin metabolites
• In vitro cytotoxicity profiling across human tumor cell lines (e.g., HeLa, A549, MCF-7)
• Crystallographic co-crystallization studies with Pol II elongation complexes
• Stability and permeability assessment in physiologically relevant membrane models (e.g., PAMPA, Caco-2)

Basic Information

Product Name	Amaninamide, S-Deoxo-Abu(1)-Ile(3)-
CAS No.	129274-06-2
Molecular Formula	C43H60N10O12S
Molecular Weight	933.07 g/mol
Synonyms	S-Deoxo-Abu(1)-Ile(3)-amaninamide; Amaninamide [S-Deoxo-Abu1,Ile3]; SD-AI-Amaninamide; Deoxy-Abu1-Ile3-amaninamide; Cyclo[Trp-Cys-Asn-Pro-Val-Cys-Asn-Ile]; α-Amaninamide derivative; S-Deoxo-Abu¹-Ile³-α-amaninamide; Amaninamide analog 129274-06-2
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches are verified for identity (IR, HPLC retention time match), purity (≥98.5% by HPLC), and absence of critical impurities (e.g., residual solvents, heavy metals, endotoxins). Testing adheres to ICH Q2(R2) guidelines and supports compliance with GMP-like documentation practices for research-grade peptides.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent oxidation and moisture uptake. Due to its hygroscopic nature, minimize exposure to ambient humidity during handling. Allow to equilibrate to room temperature in the sealed container before opening.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	98.5% – 101.0%
Related Substances (HPLC)	Single impurity ≤ 0.5%; Total impurities ≤ 1.5%
Water Content (Karl Fischer)	≤ 5.0%
Residual Solvents (GC)	MeOH ≤ 3000 ppm; ACN ≤ 410 ppm; DCM ≤ 600 ppm
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.