Description

Succinyl-Phenylalanyl-Leucyl-Phenylalanine-4-Methoxynaphthylamide is a specialized synthetic peptide substrate designed for high-precision enzymatic research. This compound is critical for studying protease activity, particularly elastase and related serine proteases, due to its specific cleavage site. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers requiring reliable and sensitive biochemical tools for kinetic assays and inhibitor screening.

Application

• Protease Enzyme Assays: Acts as a fluorogenic substrate for the continuous measurement of human leukocyte elastase (HLE) and other serine proteases.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic applications.
• Biochemical Research: Serves as a key reagent in kinetic studies to determine enzyme specificity, catalytic efficiency (kcat/Km), and inhibition constants (Ki).
• Diagnostic Development: Employed in the development of in vitro diagnostic (IVD) assays for detecting protease activity linked to inflammatory diseases.
• Quality Control in Biologics: Used to monitor the presence and activity of specific proteases during the production and purification of biopharmaceuticals.

Basic Information

Item	Details
Product Name	Succinyl-Phenylalanyl-Leucyl-Phenylalanine-4-Methoxynaphthylamide
CAS No.	129219-63-2
Molecular Formula	C41H49N5O8
Molecular Weight	739.87 g/mol
Synonyms	Suc-Phe-Leu-Phe-4-Methoxy-β-naphthylamide; Suc-F-L-F-MNA; Suc-Phe-Leu-Phe-MNA; Succinyl-Phe-Leu-Phe-4-Methoxy-2-Naphthylamide; Elastase Substrate IV; HLE Substrate; Fluorogenic Elastase Substrate; N-Succinyl-L-phenylalanyl-L-leucyl-L-phenylalanine 4-methoxy-2-naphthylamide
EINECS	Contact for details

Quality Control

Our Succinyl-Phenylalanyl-Leucyl-Phenylalanine-4-Methoxynaphthylamide is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards required for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol
Functional Activity	Conforms to reference standard in elastase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.