Description

Tyrosyl-Arginyl-Glycyl-4-Nitrophenylalanyl-Prolinamide is a synthetic peptide sequence of significant interest in biochemical research and pharmaceutical development. This compound matters as a specialized substrate or intermediate for studying enzyme kinetics, particularly for proteases like thrombin and trypsin, due to the chromogenic 4-nitrophenylalanine moiety. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in high-throughput screening, assay development, and peptide-based drug discovery.

Application

• Enzyme Substrate for Protease Assays: Used as a chromogenic substrate to measure the activity of serine proteases (e.g., thrombin, trypsin, plasmin) in biochemical research.
• Diagnostic Reagent Manufacturing: Serves as a key component in the production of diagnostic kits for coagulation disorders and other clinical assays.
• Pharmaceutical R&D: Acts as an intermediate or reference standard in the development of peptide-based therapeutics and enzyme inhibitors.
• Biochemical Research Tool: Employed in academic and industrial laboratories for studying enzyme mechanisms, specificity, and inhibition kinetics.
• Quality Control in Biologics: Utilized as a standard to validate the activity and purity of enzyme preparations used in biomanufacturing.

Basic Information

Product Name	Tyrosyl-Arginyl-Glycyl-4-Nitrophenylalanyl-Prolinamide
CAS No.	127627-13-8
Molecular Formula	C36H50N12O10
Molecular Weight	810.87 g/mol
Synonyms	Tyr-Arg-Gly-4-NO2-Phe-Pro-NH2; YRG-4-Nitrophenylalanine-P-NH2; Chromogenic Substrate H-D-Tyr-Gly-Arg-pNA AcOH; Thrombin Substrate I; Boc-D-Tyr-Gly-Arg-pNA; H-D-Tyr-Gly-Arg-p-nitroanilide acetate salt; pNA-based peptide substrate; Protease substrate 127627-13-8
EINECS	Contact for details

Quality Control

Our Tyrosyl-Arginyl-Glycyl-4-Nitrophenylalanyl-Prolinamide is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.