Description

Sulphosuccinimidyl-6-(Biotinamido)Hexanoate is a heterobifunctional crosslinking reagent designed for the biotinylation of primary amines in proteins, peptides, and other biomolecules. This compound is critical for creating stable, high-affinity conjugates used in advanced detection and purification systems. It is an essential tool for researchers and manufacturers in the fields of proteomics, immunoassay development, and diagnostic kit production.

Application

• Protein and Antibody Biotinylation: Primary application for labeling antibodies, enzymes, and other proteins for use in ELISA, Western blotting, and flow cytometry.
• Affinity Chromatography Probe Synthesis: Preparation of biotinylated ligands for immobilization onto streptavidin/avidin resins.
• Diagnostic Assay Development: Key reagent in the manufacturing of lateral flow tests, chemiluminescent immunoassays (CLIA), and other point-of-care diagnostics.
• Cell Surface Labeling: For tagging cell surface proteins and receptors for imaging, sorting, and tracking studies.
• Pull-Down Assays: Creating biotinylated baits for the isolation and identification of interacting proteins or nucleic acids.
• Microarray and Biosensor Fabrication: Functionalizing surfaces with biotin for the controlled attachment of biomolecules.

Basic Information

Product Name	Sulphosuccinimidyl-6-(Biotinamido)Hexanoate
CAS No.	127062-22-0
Molecular Formula	C20H30N4O8S
Molecular Weight	486.54 g/mol
Synonyms	Sulfo-NHS-LC-Biotin; NHS-LC-Biotin (Sulfosuccinimidyl ester); N-Hydroxysulfosuccinimide-LC-Biotin; Biotinamidohexanoic acid sulfo-N-hydroxysuccinimide ester; Biotin-XX, SSE; EZ-Link Sulfo-NHS-LC-Biotin; 6-((6-(Biotinamido)hexanoyl)amino)hexanoic acid sulfo-N-hydroxysuccinimide ester; Sulfo-NHS ester of biotinamidohexanoic acid
EINECS	Contact for details

Quality Control

Our Sulphosuccinimidyl-6-(Biotinamido)Hexanoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements for life science research and in-vitro diagnostic (IVD) manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.