Description

n-Succinyl-Ile-Ala 7-Amido-4-Methylcoumarin is a fluorogenic peptide substrate designed for the sensitive detection and kinetic analysis of proteolytic enzyme activity. This compound is of critical importance in biochemical research and pharmaceutical development for studying enzyme mechanisms and screening inhibitors. It is primarily needed by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for high-throughput assays and diagnostic applications.

Application

• Protease Activity Assays: A sensitive fluorogenic substrate for continuous monitoring of serine protease activity, including chymotrypsin and subtilisin.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Enzyme Kinetics Studies: Enables precise determination of kinetic parameters (Km, Vmax, kcat) for mechanistic enzymology research.
• Diagnostic Reagent Component: Employed in the development of in vitro diagnostic kits for detecting specific protease biomarkers.
• Biochemical Research Tool: Essential for studying protease function in cellular processes, signal transduction, and disease pathways.
• Quality Control Testing: Used in biomanufacturing to assess the purity and activity of enzyme preparations.

Basic Information

Product Name	n-Succinyl-Ile-Ala 7-Amido-4-Methylcoumarin
CAS No.	126103-95-5
Molecular Formula	C₂₆H₃₄N₄O₈
Molecular Weight	530.57 g/mol
Synonyms	Suc-Ile-Ala-AMC; Succinyl-Isoleucine-Alanine 7-Amido-4-Methylcoumarin; N-Succinyl-L-Isoleucyl-L-Alanine 7-Amido-4-Methylcoumarin; Suc-Ile-Ala-AFC (common alternate naming); Fluorogenic Substrate for Proteases; AMC-based Protease Substrate; Suc-IA-AMC; Protease Substrate VI
EINECS	Contact for details

Quality Control

Our n-Succinyl-Ile-Ala 7-Amido-4-Methylcoumarin is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your compliance and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.