Description

Fmoc-[15N]Phe-Oh is a stable isotope-labeled derivative of phenylalanine, specifically incorporating the 15N isotope into the amino acid backbone. This product is essential for advanced research and development in fields requiring precise molecular tracking and quantification. It is primarily utilized by pharmaceutical researchers, academic institutions, and contract research organizations specializing in proteomics, metabolic studies, and the synthesis of isotopically labeled peptides for use as internal standards in mass spectrometry.

Application

• Mass Spectrometry Internal Standards: Critical for the accurate quantification of peptides and proteins in complex biological matrices using LC-MS/MS.
• Proteomics Research: Used in metabolic labeling (e.g., SILAC) to study protein expression, turnover, and post-translational modifications.
• Pharmaceutical Development: Synthesis of isotopically labeled drug candidates or metabolites for pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies.
• NMR Spectroscopy: Serves as a building block for preparing peptides with 15N labels to simplify and enhance nuclear magnetic resonance spectral analysis.
• Biochemical Tracing: Enables the tracing of nitrogen metabolism pathways in cellular and microbiological systems.
• Peptide Synthesis: A key protected amino acid for solid-phase peptide synthesis (SPPS) where an 15N-labeled sequence is required.

Basic Information

Product Name	Fmoc-[15N]Phe-Oh
CAS No.	125700-32-5
Molecular Formula	C24H2115NNO4
Molecular Weight	403.44 g/mol
Synonyms	Fmoc-L-phenylalanine-15N; N-(9-Fluorenylmethoxycarbonyl)-L-phenylalanine-15N; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]-3-phenylpropanoic-15N acid; Fmoc-Phe(15N)-OH; Fmoc-15N-Phe-OH; Nα-Fmoc-L-phenylalanine-15N; 9-Fluorenylmethyloxycarbonyl-L-phenylalanine-15N
EINECS	Contact for details

Quality Control

Every batch of Fmoc-[15N]Phe-Oh is manufactured and tested under strict quality systems. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive analytical applications. A Certificate of Analysis (COA) detailing isotopic enrichment (typically >98 atom % 15N), chemical purity (by HPLC), and specific rotation is available for each lot. Our quality commitment supports compliance in GLP and GMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % 15N
Specific Rotation	-15.0° to -18.0° (c=1, DMF)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.