Description

γ-tert-Butyloxy-Glu-Gly-Arg-3-Carboxy-4-Hydroxyanilide is a specialized synthetic peptide derivative, primarily utilized as a chromogenic substrate in enzymatic assays. Its core value lies in its high specificity and sensitivity for detecting and quantifying the activity of key proteolytic enzymes, such as thrombin and trypsin, in research and diagnostic applications. This compound is essential for pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on hemostasis, proteomics, and enzyme kinetics. The product is supplied to exacting standards to ensure reliable and reproducible results in critical biochemical analyses.

Application

• Chromogenic Substrate for Enzyme Assays: Primary use as a sensitive and specific substrate for measuring the activity of serine proteases like thrombin, factor Xa, and trypsin in vitro.
• Diagnostic Kit Manufacturing: A key raw material in the production of clinical diagnostic kits for coagulation disorders, liver function, and pancreatitis.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) and kinetic studies to identify and characterize novel enzyme inhibitors for drug discovery.
• Biochemical Research: Essential tool in academic and industrial labs studying enzyme mechanisms, specificity, and regulation within proteolytic cascades.
• Quality Control in Biologics: Employed to monitor and validate the purity and activity of enzyme preparations used in therapeutic manufacturing.

Basic Information

Product Name	γ-tert-Butyloxy-Glu-Gly-Arg-3-Carboxy-4-Hydroxyanilide
CAS No.	125319-05-3
Molecular Formula	C26H40N8O9
Molecular Weight	608.65 g/mol
Synonyms	Boc-Glu-Gly-Arg-pNA; t-Boc-Glu-Gly-Arg-p-nitroanilide; Boc-E-G-R-pNA; Chromogenic Substrate Boc-E-G-R-pNA; Nα-t-Butyloxycarbonyl-L-glutamyl-glycyl-L-arginine 4-nitroanilide acetate salt; Thrombin Substrate I (Boc-based); Protease Substrate; 4-Nitroaniline derivative of Boc-Glu-Gly-Arg
EINECS	Contact for details

Quality Control

Our γ-tert-Butyloxy-Glu-Gly-Arg-3-Carboxy-4-Hydroxyanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing to ensure performance as a chromogenic substrate. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to meet specific customer or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Assay (Functional Activity)	≥ 90% of reference standard
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.