Description

Dihydroxyboronyl-Sepharose CAS NO 125022-86-8 is a specialized affinity chromatography medium designed for the selective capture and purification of cis-diol-containing biomolecules. This product is critical for achieving high-purity separations in complex biological mixtures, offering superior binding specificity and capacity. It is an essential tool for researchers and process developers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable and efficient purification of glycoproteins, nucleotides, carbohydrates, and other valuable biomolecules.

Application

• Affinity Chromatography for Glycoprotein Purification: Selective isolation of glycosylated proteins, antibodies, and enzymes from cell culture supernatants or fermentation broths.
• Nucleotide and RNA Capture: Effective purification of RNA, ATP, and other nucleotides bearing cis-diol groups for molecular biology and therapeutic applications.
• Carbohydrate and Glycan Analysis: Enrichment of sugars, oligosaccharides, and glycans for structural analysis and biomarker discovery.
• Diagnostic Reagent Manufacturing: Used in the production of diagnostic kits that require highly purified, specific biomolecular components.
• Process Development and Scale-up: Serves as a key media for developing and scaling cGMP-compliant downstream purification processes for biologics.
• Lectins and Sugar-Binding Protein Studies: A valuable tool for studying carbohydrate-protein interactions in biochemical research.

Basic Information

Product Name	Dihydroxyboronyl-Sepharose
CAS No.	125022-86-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Boronic Acid Sepharose; Phenylboronic Acid Agarose; Dihydroxyboryl-Sepharose; Boric Acid Gel; m-Aminophenylboronic Acid–Sepharose; PBA-Sepharose; Boronate Affinity Gel; cis-Diol Specific Affinity Medium
EINECS	Contact for details

Quality Control

Our Dihydroxyboronyl-Sepharose is manufactured under a strict quality management system. Each batch is tested for critical parameters including ligand density, binding capacity, particle size distribution, and flow characteristics to ensure consistent, high-performance results. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in the supplied storage solution (typically 20% ethanol) to prevent microbial growth and maintain chromatographic performance. Do not freeze. For long-term storage, ensure the container is sealed to prevent evaporation.

Specification

Item	Specification
Matrix	Cross-linked 4% or 6% Agarose (Sepharose)
Ligand	m-Aminophenylboronic Acid
Ligand Density	10 - 30 µmol/mL gel
Binding Capacity (for RNA)	>1 mg/mL gel
Particle Size	45 - 165 µm
Maximum Flow Rate	300 cm/h
pH Stability (long term)	2 - 12
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.