Description

Z-Val-Val-Arg-Amc is a fluorogenic peptide substrate designed for the sensitive detection and quantification of enzyme activity. This compound is of significant value in biochemical research and pharmaceutical development, where precise measurement of protease function is critical. It is primarily needed by researchers and quality control laboratories in the life sciences, biotechnology, and drug discovery sectors for high-throughput screening and enzymatic assay development.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the activity of various proteases, including trypsin and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors.
• Biochemical Research: Essential tool for studying enzyme kinetics, specificity, and catalytic mechanisms in vitro.
• Diagnostic Development: Serves as a core component in developing clinical diagnostic kits for conditions linked to abnormal protease levels.
• Quality Control in Biologics: Employed to monitor and ensure the consistency and activity of enzyme-based biopharmaceutical products.

Basic Information

Product Name	Z-Val-Val-Arg-Amc
CAS No.	124485-41-2
Molecular Formula	C30H46N8O7
Molecular Weight	630.74 g/mol
Synonyms	Benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin; Z-VVR-AMC; Z-Val-Val-Arg-AMC; Cbz-Val-Val-Arg-AMC; Nα-CBZ-L-Valyl-L-valyl-N5-[(benzyloxy)carbonyl]-Nω-nitro-L-arginine 7-amido-4-methylcoumarin; Boc-Val-Val-Arg-AMC (common alternative); Fluorogenic Protease Substrate VVR-AMC; Trypsin Substrate Z-VVR-AMC
EINECS	Contact for details

Quality Control

Our Z-Val-Val-Arg-Amc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.