Description

Hematoporphyrin-Sepharose CAS NO 124399-49-1 is a specialized affinity chromatography medium where hematoporphyrin is covalently immobilized onto a Sepharose matrix. This product is engineered for the selective purification and isolation of proteins and biomolecules that exhibit specific binding affinity to porphyrin structures. It is a critical tool for researchers and process developers in biopharmaceutical manufacturing, academic biochemistry research, and diagnostic assay development, enabling high-purity downstream processing.

Application

• Affinity Chromatography: Primary matrix for the purification of heme-binding proteins, certain enzymes, and receptors.
• Biopharmaceutical Downstream Processing: Isolation and purification of therapeutic proteins and antibodies in GMP-compliant processes.
• Biochemical Research: Study of protein-ligand interactions, enzyme kinetics, and binding affinity assays in laboratory settings.
• Diagnostic Kit Component: Used as a solid-phase capture medium in the development of immunoassays and other diagnostic tests.
• Sample Preparation: Pre-concentration of low-abundance target biomolecules from complex biological mixtures for proteomic analysis.

Basic Information

Product Name	Hematoporphyrin-Sepharose
CAS No.	124399-49-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Hematoporphyrin immobilized on Sepharose; Sepharose-hematoporphyrin conjugate; Hematoporphyrin-Sepharose CL-4B; Hematoporphyrin-agarose; Porphyrin affinity gel; Heme-binding Sepharose; HP-Sepharose
EINECS	Contact for details

Quality Control

Our Hematoporphyrin-Sepharose is manufactured under a controlled quality system. Each batch undergoes rigorous testing to ensure consistent performance, including assessment of ligand density, binding capacity, and matrix integrity. Certificates of Analysis (COA) detailing specific lot parameters are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in the supplied storage solution (typically 20% ethanol) to prevent microbial growth and maintain stability. Do not freeze. Keep the suspension homogeneous and avoid drying out the matrix.

Specification

Item	Specification
Matrix	Sepharose 4B (Cross-linked Agarose)
Ligand	Hematoporphyrin
Ligand Density	Contact for details
Binding Capacity	Contact for details
Particle Size	45-165 μm
Storage Solution	20% Ethanol
pH Stability	3 - 10 (operational)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.