Description

Toyopearl DEAE 650 M is a high-capacity, weak anion exchange chromatography resin designed for the purification of biomolecules. This product is essential for achieving high-resolution separation and scalable purification of proteins, nucleic acids, and other charged biomolecules in industrial processes. It is a critical material for biopharmaceutical manufacturers, academic research institutions, and diagnostic kit producers requiring reliable and reproducible downstream processing.

Application

• Downstream Bioprocessing: Primary capture and intermediate purification of monoclonal antibodies, recombinant proteins, enzymes, and vaccines.
• Nucleic Acid Purification: Isolation and purification of plasmid DNA, mRNA, and other oligonucleotides for therapeutic and research applications.
• Enzyme and Co-factor Separation: Fractionation of complex biological mixtures in enzyme production and biochemical research.
• Viral Vector and Vaccine Purification: An integral step in the purification of viral vectors for gene therapy and certain vaccine platforms.
• Diagnostic Reagent Preparation: Used in the manufacturing process of high-purity reagents for in-vitro diagnostics (IVD).
• Process Development & Scale-up: Ideal for method development in laboratory-scale columns, with direct scalability to industrial production columns.

Basic Information

Item	Detail
Product Name	Toyopearl DEAE 650 M
CAS No.	122985-81-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DEAE Toyopearl 650 M; Diethylaminoethyl Toyopearl 650 M; TSKgel DEAE-Toyopearl 650 M; Weak Anion Exchange Resin 650 M; DEAE Functionalized Polymeric Resin; Anion Exchange Chromatography Medium; 650 M Resin; DEAE-650M
EINECS	Contact for details

Quality Control

Our Toyopearl DEAE 650 M is manufactured under a rigorous quality management system. Each lot is tested to ensure consistent performance characteristics, including ligand density, particle size distribution, dynamic binding capacity, and pressure-flow properties. Certificates of Analysis (COA) documenting these specifications are available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect performance. For long-term storage, keep in the original container with the provided storage solution as per the manufacturer's instructions.

Specification

Item	Specification
Matrix	Hydrophilic Polymeric Beads
Functional Group	Diethylaminoethyl (DEAE)
Ion Exchange Type	Weak Anion Exchanger
Particle Size	~65 μm (mean)
Dynamic Binding Capacity (for BSA)	> 60 mg/mL
Maximum Operating Pressure	0.3 MPa
pH Stability	2 - 12
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.