Description

Boc-Ala-Ala-Pro-Ala-Pna CAS NO 121570-42-1 is a synthetic, protected tetrapeptide sequence featuring a para-nitroanilide (Pna) chromogenic group. This compound serves as a critical substrate for the study and characterization of serine proteases, particularly elastase and related enzymes. It is essential for researchers and manufacturers in pharmaceutical development, biochemistry, and proteomics who require high-purity, well-defined peptide substrates for kinetic assays and enzyme inhibitor screening.

Application

• Enzyme Substrate for Protease Research: Specifically designed as a chromogenic substrate for the kinetic analysis of elastase, pancreatic elastase, and other serine proteases.
• Biochemical Assay Development: Used in high-throughput screening (HTS) platforms to identify and evaluate novel protease inhibitors for therapeutic applications.
• Pharmaceutical R&D: A key tool in drug discovery programs targeting inflammatory diseases, pulmonary conditions, and certain cancers where protease activity is dysregulated.
• Diagnostic Reagent Manufacturing: Incorporated into diagnostic kits and reagents for measuring protease activity in clinical and research samples.
• Mechanistic Enzymology Studies: Enables detailed investigation of enzyme specificity, catalytic mechanism, and inhibition constants (Ki).
• Quality Control in Peptide Synthesis: Serves as a reference standard or model compound for validating synthetic methodologies and purification processes.

Basic Information

Product Name	Boc-Ala-Ala-Pro-Ala-Pna
CAS No.	121570-42-1
Molecular Formula	C27H39N7O8
Molecular Weight	589.65 g/mol
Synonyms	Boc-Ala-Ala-Pro-Ala-pNA; tert-Butyloxycarbonyl-Alanyl-Alanyl-Prolyl-Alanyl-4-nitroanilide; N-[(1,1-Dimethylethoxy)carbonyl]-L-alanyl-L-alanyl-N2-(L-prolyl)-L-alanine 4-nitroanilide; Boc-AAP-A-pNA; N-Boc-Ala-Ala-Pro-Ala-pNA; Elastase Substrate IV; AAPV-pNA; Suc-(Ala)3-pNA (related); Alanine Alanine Proline Alanine p-nitroanilide, Boc-protected
EINECS	Contact for details

Quality Control

Our Boc-Ala-Ala-Pro-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, MS for identity confirmation, and NMR for structural verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Our quality commitment supports applications requiring high batch-to-batch consistency and reliable performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.