Description

Protein Kinase C (19-31) is a synthetic peptide fragment corresponding to amino acids 19-31 of the Protein Kinase C (PKC) enzyme. This specific sequence is a critical pseudosubstrate region that plays a fundamental role in the study of PKC activation, regulation, and inhibition mechanisms. It is an essential biochemical tool for researchers in pharmacology, oncology, and neuroscience. This high-purity peptide is vital for investigating intracellular signaling pathways, developing kinase inhibitors, and conducting competitive binding assays.

Application

• Biochemical Research: Used as a substrate or inhibitor in enzymatic assays to study Protein Kinase C (PKC) activity and kinetics.
• Drug Discovery & Development: Serves as a key reagent in high-throughput screening (HTS) campaigns to identify and characterize novel PKC-targeting therapeutic compounds.
• Signal Transduction Studies: Essential for elucidating the role of PKC in cellular processes such as proliferation, differentiation, and apoptosis.
• Neuroscience Research: Applied in studies exploring PKC's involvement in neurotransmitter release, synaptic plasticity, and neurological disorders.
• Antibody Production: Can be used as an immunogen to generate specific antibodies against the PKC pseudosubstrate domain for Western blotting and immunohistochemistry.
• Cell-Based Assays: Employed in cell culture experiments to modulate PKC signaling pathways and observe downstream effects.

Basic Information

Product Name	Protein Kinase C (19-31)
CAS No.	121545-65-1
Molecular Formula	C70H122N22O19
Molecular Weight	1559.86 g/mol
Synonyms	PKC (19-31); Protein Kinase C Fragment 19-31; PKC Pseudosubstrate Peptide (19-31); RFARKGSLRQKNV; Arg-Phe-Ala-Arg-Lys-Gly-Ser-Leu-Arg-Gln-Lys-Asn-Val; H-Arg-Phe-Ala-Arg-Lys-Gly-Ser-Leu-Arg-Gln-Lys-Asn-Val-OH
EINECS	Contact for details

Quality Control

Our Protein Kinase C (19-31) is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and Mass Spectrometry (MS) for identity confirmation. Certificates of Analysis (COA) detailing purity, peptide content, and analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70%
Single Impurity (HPLC)	≤ 1.0%
Amino Acid Analysis	Within ± 10% of theoretical
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.