Description

F-Chemotactic Peptide-Fluorescein is a synthetic, fluorescein-conjugated peptide derivative designed for advanced biochemical research and diagnostic applications. This compound is critical for studying cellular chemotaxis and receptor-ligand interactions with high sensitivity due to its fluorescent tag. It is primarily utilized by researchers and developers in the fields of immunology, cell biology, and pharmaceutical discovery who require precise, traceable probes for in vitro assays and imaging studies.

Application

• Chemotaxis Assays: Used as a fluorescent tracer to study the directed migration of leukocytes and other cell types in response to chemical gradients.
• Flow Cytometry: Serves as a specific fluorescent probe for labeling and detecting cells expressing the corresponding chemotactic peptide receptor.
• Fluorescence Microscopy: Enables visualization and tracking of receptor binding and internalization processes in live or fixed cells.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify modulators of chemotactic peptide receptor activity.
• Biomarker Research: Facilitates the development of diagnostic assays targeting specific inflammatory or immune pathways.
• Receptor Binding Studies: Acts as a labeled ligand in competitive binding experiments to determine receptor affinity and specificity.

Basic Information

Product Name	F-Chemotactic Peptide-Fluorescein
CAS No.	117576-09-7
Molecular Formula	C58H73FN12O15S
Molecular Weight	1213.34 g/mol
Synonyms	N-Formyl-Met-Leu-Phe-Lys(Fluorescein); fMLFK(Fluorescein); Formyl-Methionyl-Leucyl-Phenylalanyl-Lysine(Fluorescein); Chemotactic Peptide N-Formyl-Met-Leu-Phe-Lys labeled with Fluorescein; Fluorescein labeled fMLFK; Fluorescein isothiocyanate conjugated fMLP derivative; fMLP-K(FITC); For-Met-Leu-Phe-Lys(FITC)
EINECS	Contact for details

Quality Control

Our F-Chemotactic Peptide-Fluorescein is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, peptide content, and fluorescence properties are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Fluorescein-to-Peptide Ratio (F/P)	0.8 - 1.2
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.