Description

2'-o-(tert-Butyldimethylsilyl)-5'-o-Trityluridine is a key protected nucleoside intermediate essential for the synthesis of oligonucleotides and modified RNA molecules. This compound is critical for ensuring high coupling efficiency and regioselectivity during solid-phase synthesis, directly impacting the yield and purity of the final oligonucleotide product. It is primarily required by pharmaceutical R&D laboratories, biotechnology companies, and CROs engaged in the development of antisense therapeutics, siRNA, and diagnostic probes.

Application

• Oligonucleotide Synthesis: Serves as a crucial building block in the automated solid-phase synthesis of RNA and modified oligonucleotides.
• Antisense & siRNA Therapeutic Development: Used in the research and production of therapeutic oligonucleotides for targeted gene silencing.
• Diagnostic Probe Manufacturing: Employed in the creation of labeled probes for genetic testing and molecular diagnostics.
• Pharmaceutical Intermediate: Acts as a protected precursor in the multi-step synthesis of complex nucleoside-based active pharmaceutical ingredients (APIs).
• Biochemical Research: Utilized in academic and industrial labs for studying RNA structure, function, and metabolism.
• Scale-up GMP Production: Suitable for process development and cGMP manufacturing of oligonucleotide drugs under controlled environments.

Basic Information

Item	Detail
Product Name	2'-o-(tert-Butyldimethylsilyl)-5'-o-Trityluridine
CAS No.	117136-35-3
Molecular Formula	C34H40N2O6Si
Molecular Weight	600.79 g/mol
Synonyms	2'-O-TBDMS-5'-O-Trityluridine; 2'-O-(tert-Butyldimethylsilyl)-5'-O-trityluridine; 5'-O-Trityl-2'-O-(tert-butyldimethylsilyl)uridine; Uridine, 5'-O-trityl-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-; 2'-TBDMS-5'-Tr-Ur; TBDMS-Tr-Ur; Protected Uridine Derivative
EINECS	Contact for details

Quality Control

Every batch of 2'-o-(tert-Butyldimethylsilyl)-5'-o-Trityluridine is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the material meets the stringent requirements for oligonucleotide synthesis, including HPLC purity, NMR confirmation, and moisture content analysis. Certificates of Analysis (COA) with detailed chromatograms and spectral data are available for each lot to support your regulatory and R&D documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept sealed under an inert atmosphere (argon or nitrogen) after opening to prevent moisture absorption and decomposition. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.