Description

Biotin-Maleimide CAS NO 116919-18-7 is a high-purity, heterobifunctional crosslinking reagent that combines the strong binding affinity of biotin (vitamin B7) with the selective reactivity of a maleimide group. This compound is essential for creating stable, site-specific conjugates between biomolecules containing thiol groups and avidin or streptavidin-based detection systems. It is a critical tool for researchers and manufacturers in the fields of bioconjugation, diagnostic assay development, and targeted drug delivery, enabling precise labeling and purification of proteins, antibodies, and other therapeutic agents.

Application

• Bioconjugation and Protein Labeling: Site-specific conjugation of peptides, antibodies, or other proteins (via free cysteine thiols) to biotin for detection or immobilization.
• Diagnostic Assay Development: Preparation of biotinylated detection reagents for use in ELISA, lateral flow assays, and other immunoassay platforms.
• Affinity Purification Probes: Creation of biotin-tagged ligands or small molecules for pull-down experiments and streptavidin/avidin-based purification.
• Targeted Drug Delivery Systems: Conjugation of therapeutic payloads to targeting moieties (e.g., antibodies, ligands) for the development of antibody-drug conjugates (ADCs) or other targeted therapies.
• Cell Surface Labeling: Labeling of thiol-containing cell surface proteins for flow cytometry, microscopy, or tracking studies.
• Microarray and Biosensor Fabrication: Immobilization of biomolecules onto streptavidin-coated surfaces for high-throughput screening and sensing applications.
• Structural Biology: Crosslinking for studying protein-protein interactions or for stabilizing complexes for analysis.

Basic Information

Product Name	Biotin-Maleimide
CAS No.	116919-18-7
Molecular Formula	C19H28N4O5S
Molecular Weight	424.52 g/mol
Synonyms	N-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanamide-3-maleimidopropionic acid ester; Biotin-3-maleimidopropionamide; 3-Maleimidopropionylbiocytin; Biotin-PEG3-maleimide; Maleimide-PEG3-biotin; 1-Biotinamido-4-[4'-(maleimidomethyl)cyclohexanecarboxamido]butane (BMCC); Biotin BMCC; EZ-Link Maleimide-PEG3-Biotin
EINECS	Contact for details

Quality Control

Our Biotin-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity confirmation, NMR and MS for structural identity, and functional activity testing to ensure performance in conjugation applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all relevant specifications. Our quality commitment supports applications requiring high reliability in pharmaceutical research and in vitro diagnostic (IVD) manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.