Description

3-(n-Pyridyl-2-One)-2-Hydroxypropylagarose CAS NO 116410-72-1 is a specialized affinity chromatography medium designed for the selective purification of proteins and enzymes. Its core value lies in its engineered ligand, which provides high specificity for binding target biomolecules, enabling superior purity and yield in downstream bioprocessing. This product is essential for researchers and manufacturers in the biotechnology and pharmaceutical industries, particularly for applications in enzyme purification, antibody research, and recombinant protein production.

Application

• Affinity Chromatography for Protein Purification: Primary use for isolating specific enzymes, antibodies, or recombinant proteins from complex biological mixtures.
• Biopharmaceutical Downstream Processing: Critical step in the purification train for therapeutic proteins and monoclonal antibodies.
• Enzyme Immobilization and Study: Serves as a solid support for immobilizing enzymes to study kinetics or for use in biocatalysis.
• Research & Development: Used in academic and industrial labs for proteomics research, target identification, and biomarker discovery.
• Diagnostic Reagent Production: Purification of high-purity enzymes and proteins used as components in diagnostic kits and assays.
• Nucleic Acid Binding Protein Isolation: Potential application for purifying proteins that interact with specific nucleic acid sequences.

Basic Information

Product Name	3-(n-Pyridyl-2-One)-2-Hydroxypropylagarose
CAS No.	116410-72-1
Molecular Formula	N/A (Functionalized polymer matrix)
Molecular Weight	N/A (Bead-form chromatography resin)
Synonyms	3-(N-Pyridyl-2-one)-2-hydroxypropylagarose; Pyridyl-2-one agarose; 2-Pyridone agarose; 3-(2-Oxo-1,2-dihydropyrid-3-yl)-2-hydroxypropylagarose; 3-(2-Pyridonyl)-2-hydroxypropylagarose; Affinity chromatography resin; Pyridone-agarose conjugate; 3-(2-Pyridone)-2-hydroxypropylagarose
EINECS	Contact for details

Quality Control

Our 3-(n-Pyridyl-2-One)-2-Hydroxypropylagarose is manufactured under strict quality management systems. Each batch undergoes rigorous testing for key performance parameters including ligand density, binding capacity, particle size distribution, and flow characteristics to ensure consistency and reliability for critical separations. Certificates of Analysis (COA) documenting these specifications are available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (in a refrigerator) in the supplied storage solution (typically 20% ethanol) to prevent microbial growth and maintain chromatographic performance. Do not freeze. Keep away from strong bases and organic solvents as indicated by its chemical properties.

Specification

Item	Specification
Appearance	White to off-white slurry
Matrix	Cross-linked 6% agarose beads
Ligand	3-(n-Pyridyl-2-One)-2-Hydroxypropyl
Particle Size	45-165 μm (wet)
Binding Capacity	≥ 10 mg target protein / mL resin (example)
Storage Solution	20% Ethanol
pH Stability	3 - 10 (operational)
Maximum Pressure	0.3 MPa
Microbial Bioburden	Absent

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.