Description

6'-Dimethyl-4'-(n-Succinimidyloxycarbonyl)Phenyl-10-Methyl-Acridinium-9-Carboxylate Methosulfate (Dmae-Nhs) is a highly reactive, water-soluble labeling reagent designed for the chemiluminescent detection of biomolecules. This compound is critical for researchers and manufacturers requiring high-sensitivity, non-radioactive detection systems in diagnostic and analytical applications. It is primarily utilized in the development of immunoassays, nucleic acid probes, and other bioanalytical kits within the pharmaceutical, biotechnology, and clinical diagnostics industries.

Application

• Chemiluminescent Immunoassays (CLIA): As a key labeling reagent for antibodies and antigens in automated and manual diagnostic test kits.
• Nucleic Acid Labeling: For the covalent attachment to oligonucleotide probes used in hybridization assays and molecular diagnostics.
• Protein Conjugation & Detection: Enables the sensitive labeling of proteins for Western blotting, ELISA, and other protein analysis techniques.
• Biomarker Research: Used in the development of high-throughput screening assays for disease biomarker discovery and validation.
• In Vitro Diagnostics (IVD): A core component in the manufacture of reagents for clinical chemistry analyzers and point-of-care testing devices.
• Life Science Research: Provides a stable, high-quantum-yield label for tracking and quantifying biological interactions in research settings.

Basic Information

Product Name	6'-Dimethyl-4'-(n-Succinimidyloxycarbonyl)Phenyl-10-Methyl-Acridinium-9-Carboxylate Methosulfate
CAS No.	115853-74-2
Molecular Formula	C29H27N3O7S
Molecular Weight	561.61 g/mol
Synonyms	DMAE-NHS; N-Hydroxysuccinimide ester of DMAE; 10-Methyl-9-(N-succinimidyloxycarbonylmethyl)acridinium-2-carboxylate methosulfate; Acridinium NHS ester; Chemiluminescent labeling reagent NHS ester; Acridinium-9-carboxylate, 6'-dimethyl-4'-(N-succinimidyloxycarbonyl)phenyl-10-methyl-, methosulfate; Lumi4-Tb cryptate NHS ester (related technology)
EINECS	Contact for details

Quality Control

Our DMAE-NHS is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for diagnostic and research applications. A detailed Certificate of Analysis (COA) documenting purity, performance in conjugation, and absence of critical impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Conjugation Efficiency	Functional performance tested
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.