Description

D-Phe-Pip-Arg-Pna CAS NO 115388-96-0 is a high-purity chromogenic peptide substrate specifically designed for the selective and sensitive detection of thrombin activity. This compound is critical for research and diagnostic applications requiring precise enzymatic measurement. It is primarily utilized by professionals in the pharmaceutical, biotechnology, and clinical diagnostics industries for assay development, enzyme kinetics studies, and quality control testing of coagulation factors.

Application

• Thrombin Activity Assays: Primary substrate for colorimetric and fluorometric determination of thrombin concentration and activity in research and diagnostic kits.
• Coagulation Research: Used in studies of blood coagulation pathways, inhibitor screening, and the investigation of thrombotic disorders.
• Pharmaceutical Development: Essential tool for the quality control and potency testing of heparin, direct thrombin inhibitors, and other anticoagulant drugs.
• Diagnostic Manufacturing: A key component in the production of in-vitro diagnostic (IVD) reagents for hemostasis testing panels.
• Enzyme Kinetics: Employed to study the catalytic mechanism and inhibition kinetics of thrombin and related serine proteases.
• High-Throughput Screening (HTS): Suitable for automated screening platforms to identify novel thrombin modulators for drug discovery.

Basic Information

Product Name	D-Phe-Pip-Arg-Pna
CAS No.	115388-96-0
Molecular Formula	C30H40N8O5
Molecular Weight	592.70 g/mol
Synonyms	D-Phenylalanyl-pipecolyl-arginyl-p-nitroanilide; D-Phe-Pro-Arg-pNA (alternative abbreviation); Chromogenic Substrate S-2238; H-D-Phe-Pip-Arg-pNA; Thrombin Substrate I (Chromogenic); (2S)-5-[[(2S)-1-[[(2S)-2-[[(2R)-2-Amino-3-phenylpropanoyl]amino]piperidine-1-carbonyl]-4-methylpentyl]amino]-2-[[4-nitrophenyl]amino]-5-oxopentanoic acid; Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride (related compound); Tosyl-Gly-Pro-Arg-p-nitroanilide acetate (related compound)
EINECS	Contact for details

Quality Control

Our D-Phe-Pip-Arg-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Optical Rotation	Contact for details
Absorbance Spectrum	Conforms to standard
Functional Activity	≥ 95% of reference standard in thrombin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.