Description

H-Gly-Pro-Amc Hbr is a specialized peptide derivative and fluorogenic substrate used extensively in biochemical research and pharmaceutical development. This compound is critical for the study and detection of specific enzymatic activity, particularly dipeptidyl peptidase IV (DPP-IV) and related proteases. It serves as an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors requiring precise, sensitive assay components.

Application

• Enzymatic Assay Substrate: Primary use as a fluorogenic substrate for the continuous assay of DPP-IV (CD26) activity.
• Biochemical Research: Tool compound for studying protease kinetics, inhibition, and enzyme mechanism in vitro.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and characterize potential DPP-IV inhibitors for drug discovery programs targeting type 2 diabetes and other conditions.
• Diagnostic Reagent Development: Key component in developing clinical diagnostic kits for measuring DPP-IV levels in biological samples.
• Cell Biology Studies: Application in flow cytometry and fluorescence microscopy to probe for DPP-IV activity on cell surfaces.
• Quality Control: Used as a reference standard in the QC of therapeutic proteins and enzyme preparations.

Basic Information

Product Name	H-Gly-Pro-Amc Hbr
CAS No.	115035-46-6
Molecular Formula	C17H21BrN4O4
Molecular Weight	425.28 g/mol
Synonyms	Gly-Pro-AMC HBr; H-Gly-Pro-AMC Hydrobromide; Glycyl-prolyl-4-methylcoumaryl-7-amide Hydrobromide; DPP-IV Substrate; DPP-IV Fluorogenic Substrate; GP-AMC HBr; Dipeptidyl Peptidase IV Substrate; 7-Amido-4-methylcoumarin derivative of Gly-Pro; Gly-Pro-7-amido-4-methylcoumarin HBr
EINECS	Contact for details

Quality Control

Our H-Gly-Pro-Amc Hbr is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. For specific purity requirements (e.g., >98%, >99%), please contact our technical team.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.