Description

Hirudin Hv2 CAS NO 114265-28-0 is a highly specific and potent direct thrombin inhibitor, a recombinant form of the natural anticoagulant found in medicinal leeches. This compound is critical for applications requiring precise and reversible inhibition of thrombin activity in research and development. It is an essential biochemical tool for scientists and manufacturers in the pharmaceutical and diagnostic industries, particularly for studying coagulation pathways, developing antithrombotic therapies, and creating in vitro diagnostic assays.

Application

• Biochemical Research: Used as a specific inhibitor in enzymatic studies to investigate the role of thrombin in coagulation, inflammation, and cell signaling pathways.
• Pharmaceutical Development: Serves as a reference standard and active pharmaceutical ingredient (API) in the R&D of novel anticoagulant and antithrombotic drugs.
• Diagnostic Reagents: A key component in the formulation of control plasmas, calibrators, and test kits for clinical coagulation analyzers to measure thrombin time and other hemostasis parameters.
• Medical Device Coating: Employed in research for biocompatible surface modifications on stents, catheters, and extracorporeal circuits to prevent thrombus formation.
• Cell Culture & Proteomics: Utilized to prevent fibrin clot formation in cell-based assays and to inhibit thrombin during protein sample preparation.

Basic Information

Product Name	Hirudin Hv2
CAS No.	114265-28-0
Molecular Formula	C181H263N49O55
Molecular Weight	Approx. 6963.5 g/mol
Synonyms	Recombinant Hirudin Variant 2; Hirudin HV2; r-Hirudin; Hirudin (recombinant); Thrombin inhibitor Hirudin; Leeches Hirudin; Hirudin from Hirudo medicinalis (recombinant); Antithrombin Hirudin
EINECS	Contact for details

Quality Control

Our Hirudin Hv2 is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, peptide mapping for identity confirmation, and biological activity assays to ensure consistent potency. Certificates of Analysis (COA) detailing purity, specific activity, endotoxin levels, and other critical parameters are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Specific Activity	≥ 12,000 ATU/mg
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Biological Potency	90% - 110% of stated value

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.