Description

L-Arginine 7-Amido-4-Methylcoumarin Dihydrochloride is a high-purity, fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of protease activity, particularly trypsin and trypsin-like enzymes. It is an essential reagent for the pharmaceutical, biotechnology, and diagnostic industries, enabling advanced research in enzymology, drug discovery, and biochemical pathway analysis.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and kinetic analysis of trypsin and related serine proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: A key tool for studying enzyme mechanisms, specificity, and regulation in academic and industrial R&D settings.
• Diagnostic Kit Development: Incorporated into in-vitro diagnostic (IVD) reagents for clinical testing of pancreatic function and other protease-related conditions.
• Quality Control in Biologics: Employed in purity and potency testing of therapeutic enzymes and biopharmaceutical products.
• Cell Biology Studies: Used to monitor protease activity in cell cultures and tissue samples for research in physiology and pathology.

Basic Information

Product Name	L-Arginine 7-Amido-4-Methylcoumarin Dihydrochloride
CAS No.	113712-08-6
Molecular Formula	C20H28N6O4 • 2HCl
Molecular Weight	505.40 g/mol
Synonyms	L-Arginine-7-amido-4-methylcoumarin dihydrochloride; Arg-AMC • 2HCl; H-Arg-AMC • 2HCl; Boc-Arg-AMC • 2HCl (common precursor); 7-Amido-4-methylcoumarin-L-arginine dihydrochloride; AMC-L-Arg dihydrochloride; Fluorogenic trypsin substrate; Protease substrate V (AMC); Nα-Benzoyl-L-arginine 7-amido-4-methylcoumarin hydrochloride (related compound); (S)-2-Amino-5-guanidinopentanoic acid 7-amido-4-methylcoumarin dihydrochloride
EINECS	Contact for details

Quality Control

Our L-Arginine 7-Amido-4-Methylcoumarin Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and rigorous impurity profiling. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in water or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.